Synthesis and efficiency evaluation of RND type efflux pump inhibitors

Abstract

Salmonella enterica serovar Typhimurium is the main cause of food poisoning and is the second most common cause of bacterial diarrhea. RND-type multidrug resistance efflux pumps are the main reason of Salmonella enterica cell resistance to antibiotics. It is important to investigate the mechanism of efflux pump inhibition. The aim of our work was to synthesize small dipeptide inhibitors and to evaluate the efficiency RND-type efflux pump inhibition in S. enterica cells. In this work we present data on the electrochemical assay of accumulation of an indicator compound of efflux pumps in bacteria tetraphenylphosphonium (TPP⁺) ions. We studied effects of the synthesized RND-type efflux pump inhibitors on accumulation of TPP⁺ ions in S. enterica. Data on the effective concentrations of the inhibitors and the efficiency of inhibition of RND-type efflux pump activity will be presented.