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Type of publication: conference paper
Type of publication (PDB): Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biologija / Biology (N010)
Author(s): Lipatova, Indrė;Paulauskas, Algimantas;Radzijevskaja, Jana;Gedminas, Vaclovas
Title: Bartonella pathogens in small mammals (rodentia and insectivora) and their fleas (Siphonaptera) and ticks (Ixodidae) in Lithuania
Is part of: ECCMID 2014 : 24th European congress of clinical microbiology and infectious diseases, 10 May 2014 - 13 May 2014, Barcelona, Spain [electronic resource]. Barcelona, 2014
Extent: p. 1-2
Date: 2014
Note: Programme No. P0399
Keywords: Bartonella;Ektoparazitai;Smulkieji žinduoliai;Bartonella;Ectoparasite;Small mammals
Abstract: Objectives: The aim of this study was to detect and identify Bartonella pathogens in fleas and ticks collected from small mammals (rodentia and insectivora) in Lithuania. Methods: In our study were captured 318 rodents (including Apodemus flavicollis, Apodemus sylvaticus, Myodes glareolus, Microtus agrestis, Microtus arvalis, Microtus oeconomus and Micromys minutus) and 48 insectivores (Sorex minutus) between September 2011 and September 2012. One hundred eighty four fleas (including 4 genus: Hystrichopsylla, Ctenophthalmus, Palaeopsylla, Megabothris) and 111 ticks (Ixodes ricinus) were collected from small mammals. All samples were tested by polymerase chain reaction for the detection of Bartonella using BhCS.781p/BhCS.1137n primers. Differentiation of Bartonella species was doing by nested PCR of the ITS region and the gltA gene. A nested PCR of the ITS region was performed using WITS-F/WITS-R and Bh311-332F/Bh473-452R primers. A nested PCR of the gltA gene was performed using gltA-F2/gltA-R2 and BhCS.781F/BhCS.1137R. PCR products were purified and subjected to sequence analysis. The obtained sequences were aligned with Bartonella pathogens gene sequences registered in GenBank database. Results: Bartonella pathogens were isolated from 27% small mammals, 30% fleas and 25% pools of ticks. A nested PCR and sequencing analysis of PCR products identified Bartonella pathogens to three species which can cause disease in human. B. grahamii was detected in 41% small mammals, 38% fleas and 11% pools of ticks; B. taylorii was detected in 12% small mammals, 35% fleas and 18% pools of ticks and 4% fleas were positive for both pathogens. Also we have detected B. rochalimae in 2% flea. Conclusion: In Lithuania only B. henselae was diagnosed for patient. Fleas have previously been involved in the transmission of B. henselae infection. Our study confirms that fleas and ticks are vector of Bartonella pathogens in small mammals.[...]
Affiliation(s): Biologijos katedra
Kauno Tado Ivanausko zoologijos muziejus
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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