Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/44445
Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biologija / Biology (N010)
Author(s): Rosef, Olav;Paulauskas, Algimantas;Radzijevskaja, Jana
Title: Prevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in questing Ixodes ricinus ticks in relation to the density of wild cervids
Is part of: Acta Veterinaria Scandinavica. Copenhagen : Consilium Societatum Veterinarium Scandinavicarum, Vol. 51, no. 47 (2009)
Extent: p. 1-8
Date: 2009
Keywords: Ticks;Borrelia burgdorferi;Anaplasma phagocytophilum;Wild cervids
Abstract: Background: Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum have been considered as pathogens in animals and humans. The role of wild cervids in the epidemiology is not clear. We analyzed questing Ixodes ricinus ticks collected in spring for these pathogens from sites with high (Fjellyvar and Strm) and low density (Tjore, Hinnebu and Jomfruland) of wild cervids to study the spread of the pathogens in questing ticks. Methods: For detection of Anaplasma phagocytophilum a 77-bp fragment in the msp2 gene was used. Detection of Borrelia burgdorferi sensu lato was performed using the FL6 and FL7 primers according to sequences of conserved regions of the fla gene. The OspA gene located on the linear 49-kb plasmid was used as target in multiplex PCR for genotyping. Genospecies-specific primers were used in the PCR for Borrelia burgdorferi sensu stricto, B. afzelii and B. garinii. Results: Infection rates with Borrelia spp. were significantly lower at Fjellyvar and Strm compared to Tjore and Hinnebu; Fjellyvar vs. Tjore (chi2=20.27, pyvar vs. Hinnebu (chi2=24.04, pm vs. Tjore (chi2=11.47, p=0.0007) and Strm vs. Hinnebu (chi2=16.63, pyvar vs Hinnebu (chi2=16.27, p=0.0001); Strm vs. Tjore (chi2=13.16, p=0.0003); Strm vs. Hinnebu (chi2=34.71, pyvar vs. Tjore (chi2=3.19, p=0.0742) and Fjellyvar vs. Stm (chi2=5.06, p=0.0245). Wild cervids may serve as a reservoir for A. phagocytophilum.[...]
Internet: http://www.actavetscand.com/content/pdf/1751-0147-51-47.pdf
http://www.actavetscand.com/content/pdf/1751-0147-51-47.pdf
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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