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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Kornyšova, Olga;Owens, Paul K;Maruška, Audrius
Title: Continuous beds with vancomycin as chiral stationary phase for capillary electrochromatography
Other Title: Ištisiniai sorbentai su vankomicino chiraliniu selektoriumi skirti kapiliarinei elektrochromatografijai
Is part of: Electrophoresis. Weinheim : Wiley-VCH Verlag GmbH, 2001, Vol. 22, iss. 15
Extent: p. 3335-3338
Date: 2001
Keywords: Capillary electrochromatography;Continuous bed;Monolithic;Vancomycin chiral stationary phase
Abstract: Enantiomeric separations in capillary electrochromatography (CEC) carried out using a continuous-bed chiral stationary phase (CSP) based on the macrocyclic antibiotic, vancomycin, is presented. The continuous beds were prepared from methacryloxypropyl modified fused silica capillaries (100 m ID) byin situ copolymerization of N-(hydroxymethyl)acrylamide and piperazine diacrylamide with vinyl sulfonic acid comonomer used to introduce ionic functionality and thus a strong electroosmotic flow (EOF). The CSP was subsequently prepared by immobilizing the vancomycin stationary phase by reductive amination. Preliminary results have indicated that an extremely strong EOF is obtained in both the nonaqueous polar organic (15.2Ч10-5 cm2V-1 s-1) and the aqueous reversed-phase modes of operation (8.5Ч10-5 cm2V-1 s-1). Enantioselectivity was obtained for four racemic compounds, the best of which was in the case of thalidomide which was separated in 10 minutes with high resolution (Rs = 2.5) and efficiency (120 000 plates meter -1) values
Affiliation(s): Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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