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Type of publication: conference paper
Type of publication (PDB): Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Farmacija / Pharmacy (M003)
Author(s): Balčiūnaitė, Gabrielė;Savickienė, Nijolė;Žvirblis, Gintautas;Žvirblytė, Gitana;Ragažinskienė, Ona;Kandelinskaya, Olga;Grischenko, Helena
Title: Determination of proteins in Echinacea purpurea (L.) Moench roots and rhizomes
Is part of: Pharmaceutical sciences and pharmacy practice dedicated to 145th anniversary of prof. Petras Raudonikis : the 5th international conference, November 22, 2014 Kaunas, Lithuania : Book of abstracts / edited by Indrė Šveikauskaitė. Kaunas : Lietuvos sveikatos mokslų universitetas, 2014
Extent: p. 22-24
Date: 2014
Keywords: Echinacea;Chemistry;Echinacea;Immunology;Plant roots;Chemistry;Rhizome;Chemistry;Plant extracts;Pharmacology;Plant proteins;Analysis
ISBN: 9789955153726
Abstract: Coneflower (Echinacea purpurea (L.) Moench) is one of the mostly used medical plants worldwide. Herb and roots of Echinacea purpurea (L.) Moench are used in medicine for immunomodulatory activity(Fonsecaa et al, 2014; Dapasa et al, 2014) in the treatment and prevention of common cold, influenza and other respiratory tract infections (Hart et al, 2009). Chemical constituents of coneflower are responsible for pharmacologic effect of a plant. It contains flavonoids, caffeic acid, polysaccharides, essential oils, phenolic compounds, saponins, and glycoproteins (Bauer, 1999). There are lot of research papers published wherein coneflower plant constituents are analyzed and well characterized. Unfortunately, information about coneflower proteins and possible lectin content is vague. Aim of experiment: To extract and characterize size of protein particles in coneflower roots and rhizomes. Experiment tasks: 1. To extract and fractionate proteins from coneflower roots and rhizomes; 2. To quantify protein content in coneflower roots and rhizomes fractions; 3. To identify size of protein particles with polyacrylamide gel (PAGE) electrophoresis in coneflower roots and rhizomes fractions; Materials and methods: 1. 40 g of homogenized coneflower roots and rhizomes material (roots and rhizomes of Echinacea purpurea (L.) Moench were purchased from Botanical Garden of Vytautas Magnus University) extracted in phosphate-buffer saline (PBS) with protease inhibitors and salted with ammonium sulphate at 4̊ C temperature; 2. Proteins fractionated according to size with AKTA Purifier system, using Superdex 200 10/300 GL column, eluent was phosphate buffer, prepared of 50mM sodium phosphate. [...]
Affiliation(s): Botanikos sodas
Lietuvos sveikatos mokslų universitetas. Medicinos akademija
Vilniaus universitetas. Biotechnologijų centras
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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