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Type of publication: research article
Type of publication (PDB): Straipsnis kitose duomenų bazėse / Article in other databases (S4)
Field of Science: Biologija / Biology (N010)
Author(s): Žukauskienė, Judita;Paulauskas, Algimantas;Česonienė, Laima;Daubaras, Remigijus
Title: Genetic Structure of Isolated "Vaccinium oxycoccus" Populations in Lithuania
Is part of: Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. Warsaw : Versita, Vol. 63, no. 1-2 (2009)
Extent: p. 33-36
Date: 2009
Keywords: Genetic diversity;RAPD;Isolated populations;Vaccinium oxycoccus
Abstract: The genetic population structure of the wild Cranberry Vaccinium oxycoccus was studied using RAPDs (random amplified polymorphic DNA). During the last century, intensive peat bogs drainage, regulation of water levels, and intensive cranberry picking has caused a risk for survival of wild cranberry Vaccinium oxycoccus populations in Lithuania. Genetic variation among and within isolated V. oxycoccus populations was investigated with RAPD profiles. Fifty-six clones were sampled in four populations at the Čepkeliai, Žuvintas, Kamanos Reserves and Aukštaitijos National Park. RAPD analyses of nine primers showed 213 polymorphic loci in the samples. The polymorphism level in the Čepkeliai rezerve population was 56.34%, in Žuvintas 49.77%, in Kamanos 46.95% and in Aukštatija 43.19%. Polymorphism among populations was 100%. For the total sample group, Shannon's Information Index was 0.2 and Nei's gene diversity 0.12. The estimated total proportion of diversity among populations (GST) and gene flow (Nm) were 0.14 and 3.1, respectively. The UPGMA analyses have revealed that populations of V. oxycoccus are clearly separated into four lineages and only one Čepkeliai lineage had a homogenous haplotype. Others Žuvintas, Kamanos and Aukštatijos lineages differed from each other. One clone sampled from Aukštatija National Park (a8) had a unique haplotype
Affiliation(s): Biologijos katedra
Botanikos sodas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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