Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/39691
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dc.contributor.authorPūraitė, Irma-
dc.contributor.authorPaulauskas, Algimantas-
dc.contributor.authorRosef, Olav-
dc.contributor.authorSruoga, Aniolas-
dc.coverage.spatialLT-
dc.date.accessioned2018-10-06T15:53:19Z-
dc.date.available2018-10-06T15:53:19Z-
dc.date.issued2011-
dc.identifier.issn13920146-
dc.identifier.otherVDU02-000010761-
dc.identifier.urihttp://www.lmaleidykla.lt/publ/1392-0146/2011/1/15-19.pdf-
dc.description.abstractŠiuo metu tauriųjų elnių populiacijos Lietuvoje yra gausios ir išplitusios, tačiau jų genofondas paveiktas reintrodukcijos, perkėlimo, gyvūnų laikymo aptvaruose, fragmentacijos bei medžioklės. Šių žvėrių populiacijos yra puikus genetinių padarinių tyrimų modelis. Siekiant nustatyti tauriųjų elnių genetinę įvairovę, buvo tiriami 27 individų iš Lietuvos ir Norvegijos kepenų bei raumenų pavyzdžiai. Individai buvo tiriami APPD (atsitiktinai pagausintos polimorfinės DNR) metodika. Tauriųjų elnių genetinės įvairovės analizei buvo panaudota dešimt (ROTH-180-01 – 10) pradmenų. Elektroforezės gelių rezultatai rodo, kad polimorfinių lokusų yra 64, o fragmentų dydis – nuo 150 iki 3 000 bazių porų. Gauti fragmentų dydžiai ir skaičiai buvo suvesti į „TREECON for Windows“ kompiuterinę programą, kuria remiantis įvertintas genetinis kintamumas. Genetinis kintamumas tarp tirtų tauriųjų elnių individų varijavo nuo 0,08 iki 0,80lt
dc.description.abstractDuring the last centuries, red deer (Cervus elaphus) have survived a drastic decline in population number which has gone as far as extinction in many regions of the continent. Today’s deer population is abundant and widespread, however, affected by re-introduction and keeping isolated animals in enclosures. It is necessary to observe the genetics of these animals and to monitor changes that occur in populations as well as apply the obtained knowledge in creating and restoring the species. This study investigates the levels of genetic diversity of red deer. Muscle and liver samples were obtained from 27 individuals corresponding to 2 red deer populations from Lithuania and Norway. Samples were analyzed by RAPD (random amplified polymorphic DNA) polymerase chain reaction (PCR) using ten primers (ROTH- 180-01, ROTH-180-02, ROTH-180-03, ROTH-180-04, ROTH-180-05, ROTH-180-06, ROTH-180-07, ROTH-180-08, ROTH-180-09, ROTH-180-10). PCR products were sorted according to their size by electrophoresis in 1.7% agarose gel. Gel electrophoresis showed that there were 64 polymorphic loci with the fragment size varying from 150 to 3 000. The “TREECON for Windows” programme was used for the estimation of genetic variability. Genetic variability among the red deer separate individuals varied from 0.08 to 0.80en
dc.description.sponsorshipBiologijos katedra-
dc.description.sponsorshipVytauto Didžiojo universitetas-
dc.format.extentp. 15-19-
dc.language.isoen-
dc.relation.ispartofBiologija. Vilnius : Lietuvos mokslų akademijos leidykla, 2011, vol. 57, Nr. 1-
dc.relation.isreferencedbyZoological Record-
dc.relation.isreferencedbyCAB Abstracts-
dc.relation.isreferencedbyAcademic Search Complete (EBSCO)-
dc.relation.isreferencedbyVINITI-
dc.subjectTaurieji elniailt
dc.subjectCervus elaphuslt
dc.subjectRAPDlt
dc.subjectPCRlt
dc.subjectROTH-180lt
dc.subjectGenetinė įvairovėlt
dc.subjectRed deeren
dc.subjectCervus elaphusen
dc.subjectRAPDen
dc.subjectPCRen
dc.subjectROTH-180en
dc.subjectGenetic diversityen
dc.subject.classificationStraipsnis Clarivate Analytics Web of Science Master Journal List / Article in Clarivate Analytics Web of Science Master Journal List (S2)-
dc.subject.otherBiologija / Biology (N010)-
dc.titleGenetic diversity of Norwegian and Lithuanian red deer (Cervus elaphus) populationsen
dc.title.alternativeLietuvos ir Norvegijos tauriųjų elnių (Cervus elaphus) populiacijų genetinė įvairovėlt
dc.typeresearch article-
dcterms.bibliographicCitation13-
dc.date.updated2020-05-14T09:08Z-
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local.typeS-
item.fulltextNo Fulltext-
item.grantfulltextnone-
crisitem.author.deptBiologijos katedra-
crisitem.author.deptBiologijos katedra-
crisitem.author.deptBiologijos katedra-
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