Composition of biologically active compounds of biotechnologically modified and unmodified anise hyssop

Abstract

Collections of medicinal plants have always been and are an integral part and object of the scientific research. Therefore, currently researchers are interested in various active substances of medicinal and other plants as well as their application. Object of the research is anise hyssop (Agastache foeniculum Ktze.). It is a medicinal plant characterized by anti-microbial and anti-bacterial effects. The aim of the research is to determine the composition of biologically active compounds of biologically modified and unmodified anise hyssop and its variations during the vegetation and different harvest years. Spectrophotometric method was used to determine the total contents of phenolic compounds, flavonoids and radical-scavenging activity in diploid and polyploid forms of anise hyssop in 2011, 2012 and 2013. In diploid form of the plant maximum content of phenolic compounds was accumulated in the plant in 2012 and in polyploid form in the plant of 2013. The highest total content of flavonoids in diploid and polyploid forms of the plant was accumulated in 2012. Radical-scavenging activity in diploid form of the plant was highest in 2012 and in polyploid form it was highest in 2012 and 2013. The tendency of diploid plant was observed to accumulate phenolic compounds and reach higher radical-scavenging activity at the latter vegetation phases, for polyploid plant - at the beginning of vegetation. Both forms of plant accumulate maximum amounts of flavonoids at the beginning of vegetation. Using high efficiency liquid chromatography different harvest years were compared and different antioxidant phenolic compounds were identified - chlorogenic acid, rutin and quercitrin in 2012 using DPPH radical reaction detection, and chlorogenic acid and quercetin in 2012 and 2013, using electrochemical detection. [...]