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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Aksamitienė, Edita;Hoek, Jan B;Kholodenko, Boris;Kiyatkin, Anatoly
Title: Multi-strip Western blotting to increase quantitative data output
Is part of: Electrophoresis. Weinheim : Wiley-VCH Verlag GmbH & Co, Vol. 28, iss. 18 (2007)
Extent: p. 3163-3173
Date: 2007
Keywords: Blotting errors;High throughput;Modified Western immunoblotting;Protein electrophoresis;Quantitative analysis
Abstract: The qualitative and quantitative measurements of protein abundance and protein modification states are essential in understanding their role in diverse cellular processes. Traditional Western blotting technique, though sensitive, is prone to produce substantial errors and is not readily adapted to high-throughput technologies. We propose a modified immunoblotfing procedure, which is based on simultaneous transfer of proteins from multiple gelstrips onto the same membrane, and is compatible with any conventional gel electrophoresis system. As a result, the data output per single blotting cycle can readily be increased up to ten-fold. In contrast to the traditional "one protein detection per electrophoresis cycle", this procedure allows simultaneous monitoring of up to nine different proteins. In addition to maintaining the ability to detect picogram quantities of protein, the modified system substantially improves data accuracy by reducing signal errors by two-fold. Multistrip Western blotting procedure allows making statistically reliable side-by-side comparisons of different or repeated sets of data. Compared to the traditional methods, this approach provides a more economical, reproducible, and effective procedure, facilitating the generation of large amounts of high-quality quantifiable data
Affiliation(s): Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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