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Type of publication: conference paper
Type of publication (PDB): Tezės Clarivate Analytics Web of Science / Theses in Clarivate Analytics Web of Science (T1a)
Field of Science: Biologija / Biology (N010)
Author(s): Mildažienė, Vida;Grigienė, Jurgita;Maslauskaitė, Sandra;Banienė, Rasa
Title: The effect of calcium overload on mitochondrial NAD(P)-dependent dehydrogenases
Is part of: FEBS journal. Oxford : Blackwell Pub, Vol. 273, suppl. 1 (2006)
Extent: p. 338-338
Date: 2006
Note: 31st Congress of the Federation-of-European-Biochemical-Societies (FEBS) Istanbul, TURKEY, JUN 24-29, 2006 : meeting abstracts
Keywords: Mitochondria;Dehydrogenases;Calcium
Abstract: The response of Krebs cycle dehydrogenases to Ca2+ in physiological concentration range is well established, but little is known about Ca2+ overload induced changes in the activity of essential mitochondrial dehydrogenases. We examined whether very fast decrease in the amount of mitochondrial NAD(P)H under condition of Ca2+ overload may be caused by inhibition of Ca2+ sensitive NAD(P)-dependent dehydrogenases. Ca2+ overload (increase in extramitochondrial Ca2+ concentration from 1 lM to 10 and 30 lM) did not change the activity of mitochondrial pyruvate and 2-oxoglutarate dehydrogenases. Ca2+ inhibited NADP-dependent isocitrate dehydrogenase (ICDH) by 12% at 10 lM and 20% at 30 lM Ca2+ at subsaturating concentration of substrate. NAD-dependent ICDH was activated (21%) by increase of Ca2+ concentration from 1 lM to 10 lM. At saturating substrate concentration Ca2+ had not effect on the activity of NADP-dependent ICDH but NAD-dependent ICDH was activated: activity was higher by 43% at 10 lM Ca2+ in comparison to that at 5 nM; further increase in Ca2+ concentration did not affect the activity. The activity of NADP-ICDH was 100–350- fold higher than NAD-ICDH and increase in Ca2+ concentration substantially decreased the ratio of NADP-ICDH vs. NADICDH activity. The fact that supra-physiological Ca2+ levels moderately inhibit only NADP-ICDH cannot provide explanation for Ca2+ overload induced NAD(P)H depletion and inhibition of respiration in heart mitochondria
Affiliation(s): Gamtos mokslų fakultetas
Kauno medicinos universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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