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Type of publication: conference paper
Type of publication (PDB): Tezės Clarivate Analytics Web of Science / Theses in Clarivate Analytics Web of Science (T1a)
Field of Science: Biologija / Biology (N010)
Author(s): Žūkienė, Rasa;Čižas, Paulius;Maslauskaitė, Sandra;Banienė, Rasa;Mildažienė, Vida
Title: Effects of hyperthermia on mitochondrial respiration and NAD(P)H fluorescence
Is part of: FEBS journal. Oxford : Blackwell Pub., Vol. 273, suppl. 1 (2006)
Extent: p. 77-77
Date: 2006
Note: 31st Congress of the Federation-of-European-Biochemical-Societies (FEBS) Istanbul, Turkey, Jun. 24-29, 2006 : meeting abstracts
Keywords: Mitochondria;Hyperthermia;Fluorescence
Abstract: Hyperthermia has high potential as a cancer treatment modality. That implies the need to determine the kinetic response of mitochondria from healthy tissue to moderate heating as well. We have compared the effect of moderate heating on the respiration and NAD(P)H fluorescence in isolated rat heart and liver mitochondria incubated at various Ca2+ concentrations. The rise of temperature from 37 to 42 C caused substantial increase in the inner membrane permeability in both liver and heart mitochondria, but state 3 respiration in heart mitochondria increased by 30% whereas it decreased by 13–23% in liver mitochondria [NAD(P)H fluorescence did not changed in both cases]. The response of liver and heart mitochondria was very different in the range of temperature from 42 to 47 C. Complete uncoupling of oxidative phosphorylation and the inhibition of the respiration was observed at 47 C in isolated heart mitochondria. Respiration was completely ceased in liver mitochondria, indicating that their respiratory chain is more susceptible to higher temperature. Increase of temperature to 47 C was followed by NAD(P)H fluorescence decrease both in heart and liver mitochondria. Change of free Ca2+ concentration in incubation medium from granules by A. Galoyan. In the experiments 108 Wistar male rats of 160–200 g mass were used. CS was induced by a compression of femoral soft tissues using a special press. Common amount of calcium ions was defined using crezolphtalein spectrophotometer method. The results show the level of Ca2+ in blood after 2 h compression and 2, 4, 24 and 48 h decompression and under the influence of PRP. After 2 h compression the level of Ca2+ decreases by 21% , and during decompression period the concentration of Ca2+ increases in blood by 20%, 21%, 36%, 47%, accordingly after 2, 4, 24 and 48 h decompression. So, the decompression period after 2 h compression is characterized by the increasing level of Ca2+ in blood.[...]
Affiliation(s): Gamtos mokslų fakultetas
Kauno medicinos universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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