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Type of publication: conference paper
Type of publication (PDB): Straipsnis nerecenzuojamoje Lietuvos tarptautinės konferencijos medžiagoje / Article in non-peer-reviewed Lithuanian international conference proceedings (P2b)
Field of Science: Biologija / Biology (N010)
Author(s): Rosef, Olav;Paulauskas, Algimantas;Stolan, Aud;Bråthen, Elisabeth Moen
Title: Diversity of thermophilic Campylobacter isolated from slaughter pigs in Norway
Is part of: Aktualios kiaulių ir paukščių mitybos problemos. Produkcijos kokybė : tarptautinės mokslinės konferencijos, skirtos Lietuvos veterinarijos akademijos Gyvulininkystės katedros įkūrimo 70-osioms metinėms paminėti, Kaunas, 2008 : mokslinių pranešimų medžiaga. Kaunas : Lietuvos veterinarijos akademija, 2008
Extent: p. 101-104
Date: 2008
Keywords: Campylobacter;Diversity;Pig;Genotyping
ISBN: 9789955652267
Abstract: Of 100 faecal swab samples 88 thermophilic Campylobacter (88%) representing ten herds was isolated. The species were identified by biotyping and multiplex polymerase chain reaction (PCR). Of the isolates, 86 (97.7%) were C. coli one was C. lari and one was C. jejuni. All three species was isolated from the same herd. Automatic riboprints were performed with the PstI restriction enzyme and RiboPrinter®. The identification of the isolates was predicted when the corresponding pattern matched one of the patterns of the DuPont identification (DUP-ID) library and was then assigned an identification number. A total of 81 selected C. coli isolates were riboprinted and divided into two clades and five subclades and 19 isolates could be given five different Dup-ID from the library, DUP-PST1-1182 (n=6), DUP-PST1-1208 (n=10), DUP-PST1-1175 (n=1), DUP-PST1-1140 (n=1) and DUP-PST1-1163 (n=1). Pigs are healthy carriers of C. coli that showed a high grade of diversity between and within the herds
Affiliation(s): Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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