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Type of publication: research article
Type of publication (PDB): Straipsnis konferencijos medžiagoje Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or Scopus DB conference proceedings (P1a)
Field of Science: Biofizika / Biophysics (N011)
Author(s): Čepurnienė, Karolina;Šatkauskas, Saulius
Title: Dependence of DNA electrotransfer into cells in vitro on cell electroporation and DNA electrophoresis
Is part of: IFMBE proceedings: NBC 2008, 16–20 June 2008 Riga, Latvia. Vol. 20, 14th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. New York: Springer, 2008
Extent: p. 606-609
Date: 2008
Keywords: Electroporation;Electropermeabilization;Gene electrotransfer;Gene therapy
ISBN: 9783540693666
Abstract: Electroporation mostly refers to the technique of permeabilizing cell membranes by applying short and intense electric pulses across a cell, such that the barrier function of the membrane is temporally disturbed. This allows various molecules, including plasmid DNA to pass across the membrane and induce their biological effects. The mechanism of the translocation of plasmid DNA through the permeabilized membrane is different from simple diffusion and remains poorly understood. In this in vitro study we used two types of pulses, high voltage, short duration (HV) and low voltage short duration (LV) pulses to evaluate cell electrotransfection efficiency in dependence of cell permeabilization (HV pulse) and DNA electrophoresis (LV pulse). Our results showed that DNA electrotransfer was mainly dependent on perneabilizing HV pulse. Differently from in vivo studies, this study showed that contribution of LV pulse to DNA electrotransfer is negligible
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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