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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Chemijos inžinerija / Chemical engineering (T005);Biologija / Biology (N010)
Author(s): Damašius, Jonas;Venskutonis, Petras Rimantas;Kaškonienė, Vilma;Maruška, Audrius
Title: Fast screening of the main phenolic acids with antioxidant properties in common spices using on-line HPLC/UV/DPPH radical scavenging assay
Is part of: Analytical methods. London : Royal society of chemistry, Vol. 6, 2014
Extent: p. 2774-2779
Date: 2014
Keywords: Prieskoniai;Antioksidacinis aktyvumas;DPPH;Pokolonėlinės reakcijos detekcija;Spices;Antioxidant properties;DPPH;Post-column reaction detection
Abstract: Batch 1,1-diphenyl-2-picrylhydrazyl (DPPHc) and on-line HPLC/UV/DPPHc radical scavenging assays were compared for the rapid screening of antioxidant properties of extracts of six Lamiaceae family spices, namely Origanum vulgare, Ocimum basilicum, Rosmarinus officinalis, Origanum majorana, Thymus vulgaris and Satureja hortensis. Generally, all spices showed good radical scavenging properties, which were in the following order: oregano > rosemary > savory thyme marjoram > basil. Rosmarinic acid and lithospermic acid B, as strong antioxidants, were identified according to their retention time, UV and mass spectra. Rosmarinic acid was found to be the main compound in all extracts and as it was measured by HPLC/UV/DPPHc it was responsible for more than 50% of the total extract radical scavenging capacity, except for oregano, when rosmarinic and lithospermic acid B were almost equally important radical scavengers. The highest amounts of rosmarinic and lithospermic acids were found in oregano extracts; lithospermic acid B was also found in reasonable concentrations in marjoram, savory and thyme extracts. Lithospermic acid B is reported as a constituent of marjoram, savory and thyme for the first time
Affiliation(s): Biochemijos katedra
Kauno technologijos universitetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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