Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/37358
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dc.contributor.authorPaulauskas, Algimantas-
dc.contributor.authorRadzijevskaja, Jana-
dc.contributor.authorAmbrasienė, Daiva-
dc.contributor.authorRosef, Olav-
dc.coverage.spatialLT-
dc.date.accessioned2018-10-06T14:14:12Z-
dc.date.available2018-10-06T14:14:12Z-
dc.date.issued2008-
dc.identifier.issn13920146-
dc.identifier.otherVDU02-000005167-
dc.identifier.urihttp://www.lmaleidykla.lt/biologija/2008/3/4804-
dc.identifier.urihttp://www.lmaleidykla.lt/biologija/2008/3/4804-
dc.description.abstractSkirtingais molekuliniais tyrimo metodais (PGR, Multiplex-PGR, RT-PGR, sekvenavimas) buvo nustatyti įvairūs erkių pernešami patogenai – Laimo boreliozės, anaplazmozės ir babeziozės sukėlėjai erkėse ir graužikuose iš Lietuvos ir Norvegijos. Lietuvoje bendras erkių, surinktų nuo augalų, užkrėstumas B. burgdorferi s. l. buvo 13,3% (223/1679), Norvegijoje – 5,4% (75 /383). B. burgdorferi s. l. nustatyta 53 iš 428 (12,4%) I. ricinus erkių, rastų ant graužikų Lietuvoje, ir 30 iš 782 (3,8%) Norvegijoje. Nustatyta, kad migruojantys paukščiai perneša B. burgdorferi s. l. užsikrėtusias erkes (24 iš 173 (13,8%)). Atlikus graužikų ausų ekstraktų PGR analizę, teigiama B. burgdorferi s. l. infekcija nustatyta 68 iš 398 (17,1%) graužikų. Rastos trys žmogui patogeniškos borelijų rūšys (B. afzelii, B. garinii ir B. burgdorferi s.s.) erkėse ir graužikuose. Tag Man RT-PGR metodu nustatyta, kad Lietuvoje 2,5 % erkių, rastų ant augalų, užsikrėtusios Anaplasma phagocytophylum, o Norvegijoje – 5,7% (93 iš 1634) erkių ant augalų bei 7,1% erkių ant paukščiųlt
dc.description.abstractThe use of molecular methods such as species-specific PCR, Multiplex-PCR, RT-PCR, reverse line blot hybridisation in investigations of tick-borne pathogens allowed to detect and identify the causative agents of Lyme borreliosis, anaplasmosis, and babesiosis in ticks and rodents in Lithuania and Norway. The overall prevalence of Borrelia burgdorferi s. l. infection detected in Lithuanian and Norwegian ticks was found to be 13.3% (223/1679) and 5.4% (75 /1383), respectively. A total 68 of 398 (17.1%) rodent ear extractions screened by PCR were found positive for B. burgdorferi s. l. infection. B. burgdorferi s. l. was detected in 53 of 428 (12.4%) immature Ixodes ricinus ticks collected on rodents in Lithuania and in 30 of 782 (3.8%) collected on rodents in Norway. In 24 of 173 (13.8%) ticks feeding on passerine migrating birds collected in Norway, B. burgdorferi s. l. pathogens were detected. Three clinically important species (B. afzelii, B. garinii and B. burgdorferi s. s.) were identified in ticks and rodents. Anaplasma sp. was detected in 5% of questing ticks and 19.6% of ticks collected from birds in Lithuania. A. phagocytophilum pathogens were detected in 7.1% of ticks from birds and in 93 of 1634 (5.7%) I. ricinus ticks collected from vegetation in Norway. To Babesia divergens, positive were 2% and 0.9% of questing ticks collected in Lithuania and Norway respectivelyen
dc.description.sponsorshipVytauto Didžiojo universitetas-
dc.format.extentp. 192-197-
dc.language.isoen-
dc.relation.ispartofBiologija. Vilnius : Lietuvos mokslų akademijos leidykla, 2008, Vol. 54, nr. 3-
dc.relation.isreferencedbyZoological Record-
dc.relation.isreferencedbyCAB Abstracts-
dc.relation.isreferencedbyAcademic Search Complete (EBSCO)-
dc.relation.isreferencedbyVINITI-
dc.subjectErkių pernešami patogenailt
dc.subjectIxodes ricinuslt
dc.subjectPGRlt
dc.subjectMolekuliniai nustatymo metodailt
dc.subjectTick-borne pathogensen
dc.subjectIxodes ricinusen
dc.subjectPCRen
dc.subjectMolecular detection methodsen
dc.subject.otherBiologija / Biology (N010)-
dc.titleDetection of tick-borne pathogens by molecular methodsen
dc.title.alternativeErkių pernešamų patogenų nustatymas molekuliniais metodaislt
dc.typeStraipsnis Clarivate Analytics Web of Science Master Journal List / Article in Clarivate Analytics Web of Science Master Journal List (S2)-
dcterms.bibliographicCitation32-
dc.date.updated2011-03-25T10:35Z-
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item.grantfulltextopen-
item.fulltextWith Fulltext-
crisitem.author.deptBiologijos katedra-
crisitem.author.deptBiologijos katedra-
crisitem.author.deptBiologijos katedra-
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications
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