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Type of publication: Straipsnis / Article
Author(s): Šatkauskas, Saulius;Venckūnas, Tomas;Baltušnikas, Juozas;Kilikevičius, Audrius;Lionikas, Arimantas
Title: Plasmid electrotransfer into mouse Soleus muscle
Is part of: Biomedical engineering 2014: 18th international conference, 27-28 November, 2014, Kaunas, Lithuania, 2014, p. 59-62
Date: 2014
Keywords: Elektroporacija;Elektrotransfekcija;Genų terapija;DNR elektropernaša;Electroporation;Electrotransfection;Gene therapy;DNA electrotransfer
Abstract: Plasmid electrotransfer into mouse soleus muscle may help to assess gene effect on muscle function. The aim if this study was to evaluate transfection efficiency of superficial tibialis anterior muscle plasmid electrophoretic transfer protocol for deeper soleus muscle. Plasmid with green fluorescent protein was injected into soleus muscle and thereafter eight square-wave 20 ms and 200 V/cm electrical pulses at 1 Hz were used to induce the transfection. Transfection efficiency was ~22 % thus methodology adjustments are needed when higher level of transfection is required.
Appears in Collections:3. Konferencijų medžiaga / Conference materials

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