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Type of publication: Article in Clarivate Analytics Web of Science or / and Scopus (S1);Straipsnis Clarivate Analytics Web of Science ar/ir Scopus (S1)
Field of Science: Biology (N010);Biochemistry (N004);Biologija (N010);Biochemija (N004)
Author(s): Jarmalaitė, Sonata;Daugelavičius, Rimantas;Daniūnaitė, Kristina;Šulskytė, Indrė;Kubiliūtė, Raimonda
Title: Molecular features of doxorubicin-resistance development in colorectal cancer CX-1 cell line
Is part of: Medicina. Kaunas : Lietuvos sveikatos mokslų universitetas, 2016, T. 52, nr. 5
Extent: p. 298-306
Date: 2016
Note: eISSN 1648-9144
Keywords: Doxorubicin resistance;Epithelial-to-mesenchymal transition;Atsparumas doksorubicinui;CX-1 ląstelės;Žarnyno vėžys;Epitelinis-mezenchiminis virsmas;Colon cancer CX-1
Abstract: Background and aim: Resistance to chemotherapy is the key obstacle to the effective treatment of various cancers. Accumulating evidence suggests significant involvement of the epithelial-to-mesenchymal transition (EMT) in the chemoresistance of most cancer types. This study aimed at analyzing the gene expression profile of doxorubicin (DOX)-resistant colorectal cancer cells CX-1. Materials and methods: DOX-resistant CX-1 cell sublines were acquired by stepwise increment of DOX concentrations in cell growth media. Global gene expression profiling was performed using human gene expression microarrays. The expression levels of individual genes were assessed by means of quantitative PCR (qPCR), while the DNA methylation pattern of several selected genes was determined by methylation-specific PCR. Results: Four DOX-resistant CX-1 sublines were established as a valuable tool for cell chemoresistance studies. Altered expression of the EMT, cell adhesion and motility, and chemoresistance-related genes was observed in DOX-resistant cells by genome-wide gene expression analysis. Besides, early and significant upregulation of the key EMT genes ZEB1 (5.8 ; P < 0.001) and CDH2 (6.2 ; P = 0.044) was identified by qPCR, with subsequent activation of drug transporter gene ABCC1 (3.3 ; P = 0.007) and cell stemness gene NANOG (2.4 ; P = 0.008). Downregulation of TET1 (2.1 ; P = 0.041) and changes in the methylation status of the p16 gene were also involved in the acquisition of cell resistance to DOX
Affiliation(s): Vytauto Didžiojo universitetas
Vilniaus universitetas
Nacionalinis vėžio institutas
Gamtos mokslų fakultetas
Biochemijos katedra
Appears in Collections:1. Straipsniai / Articles
Universiteto mokslo publikacijos / University Research Publications

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