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Type of publication: Straipsnis / Article
Author(s): Samuitienė, Marija;Navalinskienė, Meletėlė
Title: Identification of viral and phytoplasmal agents responsible for diseases affecting plants of Thalictrum L. in Lithuania
Other Title: Vingirius (Thalictrum sp.) pažeidžiančių virusinių ir fitoplazminių ligų sukėlėjų identifikavimas
Is part of: Vytauto Didžiojo universiteto Botanikos sodo raštai, 2008, t. 12, p. 59-72
Date: 2008
Keywords: EM;Identification;PCR;Phytoplasma;RFLP;Test-plants;Thalictrum;Virus
Abstract: Thalictrum plants exhibiting symptoms characteristic of viral and phytoplasmal diseases were collected at botanical gardens and different floriculture farms in Lithuania. Viral disease was expressed by leaf curling, pinpoint light green spotting and malformation. The causal agent of viral disease, Tomato ringspot nepovirus was isolated and identified by the me-thods of test-plants and transmission electron microscopy. Phytoplasmal symptoms included general stunting of plants, proliferation and flower distortion. Amplification of phytoplasmal 16SrRNA gene sequence in polymerase chain reactions (PCRs) containing phytoplasma universal primer pairs P1/P7, R16F2n/R16R2 and template DNA extracted from diseased plants, confirmed that the plants were infected by phytoplasma. The 1,2 kbp rDNA product was subjected to single enzyme digestions with 9 restriction endonucleases. RFLP analysis revealed that the Thalictrum plants were infected by a phytoplasma belonging to group 16SrI (aster yellows phytoplasma group), subgroup L. This work was the first investigation of viral and phytoplasmal diseases of Thalictrum plants and identification of their causal agents. Thalictrum is the new host-plant of Tomato ringspot nepovirus (ToRSV) and subgroup 16Sr I–L of phytoplasmas.
Appears in Collections:Vytauto Didžiojo universiteto Botanikos sodo raštai 2008, [t.] 12

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