Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/33416
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dc.contributor.authorZitikaitė, Irena
dc.date.accessioned2017-01-16T12:45:41Z
dc.date.available2017-01-16T12:45:41Z
dc.date.issued2008
dc.identifier.issn2345-0215
dc.identifier.urihttps://eltalpykla.vdu.lt/1/33416
dc.identifier.urihttps://hdl.handle.net/20.500.12259/33416-
dc.description.abstractNematode-transmitted viruses cause economically important diseases in a range of crops worldwide. This study presents the results of detection of nematode-transmitted viruses isolated from some vegetable crops in Lithuania. Samples of cucumber (Cucumis sativus L.), zucchini (Cucurbita pepo L.) and tomato (Lycopersicon esculentum Mill.) crops exhibiting symptoms characteristic of viral diseases were found in various field-grown and greenhouses. Viral diseases were expressed by symptoms of mottling, yellow ringspots, vein necrosis and distortion of leaves. Growth and fruiting of plants were slightly reduced. For identification of virus disease agents traditional (transmission electron microscopy and test-plants) methods and molecular diagnostic (reverse transcription-polymerase chain reaction, RT-PCR) technique were used. Identification of causal agents was based on specific symptoms of infected vegetables, determined experimental host ranges, morphological properties of virions and on cDNA amplified product sizes of nepoviruses in PCRs using virus-specific oligonucleotides. A 499 and 420 bp specific DNA fragments were amplified using isolates from cucumber and tomato samples infected with Tomato ringspot nepovirus and Arabis mosaic nepovirus, respectively.en_US
dc.language.isoenen_US
dc.relation.ispartofVytauto Didžiojo universiteto Botanikos sodo raštai, 2008, t. 12, p. 147-163lt_LT
dc.rightsSutarties data 2009-12-29, nr. A925, laisvai prieinama internetelt_LT
dc.subjectCucumberen_US
dc.subjectIdentificationen_US
dc.subjectNepovirusesen_US
dc.subjectRT-PCRen_US
dc.subjectTomatoen_US
dc.subjectZucchinien_US
dc.titleNematode-transmissible virus disease agents detected in some vegetable cropsen_US
dc.title.alternativeKai kuriose daržovėse aptikti nematodų pernešami virusinių ligų sukėlėjailt_LT
dc.typeStraipsnis / Article
dc.subject.udc58 Botanika / Botany
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item.grantfulltextopen-
Appears in Collections:Vytauto Didžiojo universiteto Botanikos sodo raštai 2008, [t.] 12
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