Preliminary analysis of essential oil composition of Artemisia Alba Turra introduced in Lithuania

Abstract

Introduction. In recent years, interest in Artemisia L. plants has grown in various fields of treatment. The essential oils of the genus Artemisia have a broad spectrum of bioactivity. The object of investigation was Artemisia alba Turra – perennial, medicinal, aromatic plant of Asteraceae (Bercht. & J. Presl) family. Aim. The aim of this study was to investigate the qualitative and quantitative composition of essential oils obtained from Artemisia alba Turra grown under Lithuanian climatic conditions. Materials and methods. The medicinal raw material of Artemisia alba Turra were collected during massive flowering vegetation stage and dried at 25°C temperature in a ventilated lodge avoiding direct solar radiaton for 4 weeks. The dried material was stored in a dark room at ambient temperature and used for further analysis. The preparation of essential oils was done using a modified standard procedure according to the European Pharmacopoeia. The essential oils were obtained by hydro distillation using a closed type Clevenger apparatus. In brief, 25 g of the dried material was mixed with 500 mL distilled water and submitted to extraction for 3 hours. The analysis of essential oils was performed using gas chromatography with a mass spectrometer detector (GC-MS-QP2010, Shimadzu, Tokyo, Japan) equipped with a Shimadzu autoinjector AOC-5000 (Shimadzu, Tokyo, Japan). Separation of compounds was performed on a RXI-5MS column (30 m × 0.25 mm i.d.×0.25 μm film thickness) (Restec, Bellefonte, USA). The operational conditions were as follows: temperature program from 50°C (5 min) to 200°C at 2°C/min and to 315°C (15 min) at 15°C/min. The GC-MS-QP2010 was equipped with a split/splitless injector (260°C). Split ratio: 1:60. Inlet pressure: 70.2 kPa. Carrier gas: helium (purity >99%), delivered at constant linear velocity 40 cm/s. Interface temperature: 280°C. MS ionization mode: electron ionization. Detector voltage: 0,99 kV. Acquisition mass range: 29-500 u. Scan speed: 2500 amu/s. Acquisition mode: full scan, scan interval 0,20 s. Results and their discussion. A preliminary study on chemical composition of essential oils from Artemisia alba Turra were conducted in 2022. A total of 30 different compounds were identified in the essential oils. The data showed that amounts of compounds with the content of major constituents varied significantly from (0.13 to 40.17%). 1-Octen-3-ol (40.17) and cymene (19.95) have been found as a major compounds. Amount of principal compounds is listed in Table 1 and expressed in arbitrary units of peak area in GC/MS chromatogram […]