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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biologija / Biology (N010)
Author(s): Balčiūnaitė - Murzienė, Gabrielė;Miknienė, Zoja;Ragažinskienė, Ona;Juodžiukynienė, Nomeda;Savickas, Arūnas;Savickienė, Nijolė;Pangonytė, Dalia
Title: Echinacea purpurea L. (Moench) hemagglutinin effect on immune response In Vivo
Is part of: Plants-Basel. Basel : MDPI AG, 2021, vol. 10, iss. 5
Extent: p. 1-11
Date: 2021
Note: Article Number: 936
Keywords: Echinacea purpurea L. (Moench);Lectin;Hemagglutinin;LysM;Immune response;T lym-phocytes;Spleen;Blood cells
Abstract: Echinacea purpurea L. (Moench) is used in traditional and conventional medicine. However, there is lack of data on the biological activities of primary plant metabolite lectins. The aim of our experiment was to find out how lectin LysM (lysine motif), which was previously purified, affects the immune response in vivo. Eight-week-old BALB/c male mice (n = 15) received four weekly 250 mu g/kg peritonial injections of purified Echinacea purpurea L. (Moench) roots' LysM lectin. The control animal group (n = 15) received 50 mu L peritoneal injections of fresh Echinacea purpurea L. (Moench) root tincture, and the negative control animal group (n = 15) received 50 mu L peritoneal injections of physiological solution. At the fifth experimental week, the animals were sedated with carbon dioxide, and later euthanized by cervical dislocation, and then their blood and spleen samples were collected. The leukocytes' formula and lymphocytes' count was estimated in blood samples, the T lymphocytes' density was evaluated in spleen zones. A statistically significant (p < 0.05) difference between each group was observed in the leukocytes' formula (monocytes' percentage, also little, medium and giant size lymphocytes). The purple coneflower fresh roots' tincture significantly decreased (p < 0.05) the T lymphocytes' quantity in peritoneal lymphoid sheaths (PALS) compared with the physiological solution injection's group (p < 0.05) and the lectin injection's group (p < 0.001). Meanwhile, lectin injections caused a significant (p < 0.01) increase in the T lymphocytes in a spleen PALS zone, compared with the physiological solution and tincture injection's group. Our data suggests that LysM lectin acts as an immunostimulant, while fresh purple coneflower tincture causes immunosuppression
Affiliation(s): Lietuvos sveikatos mokslų universitetas. Medicinos akademija
Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija
Vaistinių ir prieskon. augalų moks. sek.
Vytauto Didžiojo universitetas
Appears in Collections:1. Straipsniai / Articles
Universiteto mokslo publikacijos / University Research Publications

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