Development of miniaturized capillary zone electrophoresis equipment for analysis of polycyclic aromatic hydrocarbons degrading enzymes

Abstract

In modern scientific research a lot of attention is devoted to miniaturization of chemical analysis equipment. Also in environmental protection research field more and more attention is devoted to development of new bioremediation methods and technologies. One of emerging problems in environmental protection field is accumulation of out of use wooden railway sleepers. To protect wood form biological degradation, wooden railway sleepers are impregnated with creosote. Polycyclic aromatic hydrocarbons (PAH\'s) form a high proportion of creosote mass. A large number of PAH\'s is potential are carcinogenic compounds. The bio - remediation technology is based upon secretion of non - specific enzymes which are capable of degrading PAH\'s, produced by white root fungi. The aim of this research is to develop fully automated capillary zone electrophoresis equipment and optimize analysis conditions for effective analysis and identification of PAH's degrading enzymes. First step in this research is develop and manufacture fully automated capillary zone electrophoresis equipment coupled with contactless conductivity detector.