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Type of publication: master thesis
Field of Science: Biologija / Biology (N010)
Author(s): Sivickis, Karolis
Title: Lietuvos smulkiųjų žinduolių užsikrėtimas erkių pernešamais patogenais
Other Title: The prevelence of tick born pathogens in small mammals in Lithuania
Extent: 49 p.
Date: 26-May-2015
Keywords: Rickettsia;Anaplasma;Borrelia;Bartonella;Babesia;Rickettsia;Anaplasma;Borrelia;Bartonella;Babesia
Abstract: Šiame darbe buvo ištirti 162 smulkieji žinduoliai norint patikrinti ar individai buvo infekuoti Rickettsiaspp., Anaplasma spp. patogenais ir Borrelias pp.116 smulkieji žinduoliai. Taip pat buvo ištirti 388 smulkieji žinduoliai ar nėra infekuoti Bartonella spp., ir Babesia spp. patogenais. Smulkieji graužikai buvo gaudomi 2013-2014 metais skirtinguose biotopuose. Individai tirti naudojant PGR metodą. Tyrimams naudoti blužnies pavyzdžiai. Tiriant ar žinduoliai buvo infekuoti Rickettsiaspp. buvo panaudoti gltA geno, Borrelia spp. ospA geno, Anaplasma spp 16s RNR geno, Bartonella spp. gltA geno ir ITS regiono, o Babesia spp. 18S RNR. geno molekuliniai žymenys.Ištiri smulkieji graužikai nebuvo infekuoti Rickettsia spp., Borrelia spp ir Anaplasma spp. patogenais. Tiriant ar smulkieji graužikai infekuoti Bartonella spp. buvo nustatyta 26 % (102 iš 388), taip pat tyrimų metu nustatyta, kad žinduoliai infekuoti Babesia spp. patogenais 6% (25 iš 388).
In this study were investigated 162 small mammals to know if they are infected of Rickettsia spp., Anaplasma spp. pathogens and also 116 small mammals of Borrelia spp pathogens. Also in this work were investigated 388 small mammals if they were infected Bartonella spp., and Babesia spp. pathogens. Small mammals were captured from 2013 to 2014 in different biotopes. Individuals were investigated using PCR reactions. For researches were used spleen samples. For identification of Rickettsia spp. were use dglt A gene molecular markers, for Borrelia spp. ospA gene, Anaplasma spp 16s RNA genE, Bartonella spp. gltA geneand ITS regione, and forBabesia spp. 18S RNA. gene molecular markers. Investigated small mammals were not infected Rickettsia spp., Borrelia spp. and Anaplasma spp. pathogens. In examined mammals of Bartonella spp. were detected 26 % (102 from 388) of contagions, also 6% examined mammals of Babesia spp. were detected 6%(25 iš 388) of contagions.
Affiliation(s): Vytauto Didžiojo universitetas
Appears in Collections:VDU, ASU ir LEU iki / until 2018

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