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Type of publication: master thesis
Field of Science: Biologija / Biology (N010)
Author(s): Paulavičiūtė, Brigita
Title: Elachista genties drugių (Lepidoptera: Elachistidae) DNR molekulinių žymenų paieška, analizė ir panaudojimas populiacijų tyrimuose
Other Title: Searching of DNA markers in Elachista genus moths (Lepidoptera: Elachistidae), their analysis and the use in population reaearch
Extent: 81 p.
Date: 18-Jun-2006
Keywords: Elachistidae;Lepidoptera;biology;drugiai;insecta;biologija;vabzdžiai;DNR.
Abstract: The chosen group - Elachistidae (Insecta: Lepidoptera) was researched only with the help of traditional research methods and the molecular DNA method was never applied. Model species Elachista argentella, E. maculicerusella and E. pollinariella. Selected populations were located in a different distance from each other. Elachista argentella,. E. maculicerusella and E. pollinariella were examined using traditional entomological methods such as identifying of species, making of morphological preparations, documenting of important for taxonomy morphological structures. DNA extraction method was applied for the above mentioned Elachista species. There were 4 specific and 2 random primers chosen for the molecular research. Analysis of Elachista argentell,. E. maculicerusella and E. pollinariella was carried out using oligonucleotide pair which amplifies mitochondrial DNA sequences of the COI gene. The polymorphism of sequenced mtDNA segments of the COI gene in three Elachista genus species was assessed on species and population level.
Affiliation(s): Švietimo akademija
Vytauto Didžiojo universitetas
Appears in Collections:VDU, ASU ir LEU iki / until 2018

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