Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorNavickaitė, Diana-
dc.contributor.authorRuzgys, Paulius-
dc.contributor.authorMaciulevičius, Martynas-
dc.contributor.authorSincevičiūtė, Rūta-
dc.contributor.authorŠatkauskas, Saulius-
dc.description.abstractElectroporation is a method, developed in order to deliver exogenous biocompounds (anticancer drugs, nucleic acids, proteins, etc.) to cells and tissues, which are mostly designed for cancer treatment purposes. An alternative to chemotherapeutic drugs can be Ca2+ ions which are universal signal messengers that regulate a multitude of cellular functions. The application of calcium electroporation is a novel anticancer treatment that selectively kills cancer cells by necrosis, a cell death pathway that can induce the immune system due to the high release of antigens. However, the mechanism behind the calcium electroporation remains unclear to this day. Calcium ions are also known to play a key role in membrane resealing, potentially altering the pore dynamics and molecular delivery during electroporation. To elucidate the role of calcium ions in the process of electroporation, we used different extracellular calcium concentrations with different fluorescent dyes (Propidium iodide (PI), YO-PRO-1 and ethidium bromide (EtBr) in electroporation media. Experiments were performed using flow cytometry and fluorescent microscopy methods. In conclusion, we report that extracellular calcium induces a negative effect to the small molecule (Propidium iodide (PI), YO-PRO-1 and ethidium bromide (EtBr), transfer into the cells after electroporation. Keywords: calcium electroporation, microsecond electroporation, calcium, PI electrotransfer, pore resealing, membrane repairen
dc.description.sponsorshipBiologijos katedra-
dc.description.sponsorshipGamtos mokslų fakultetas-
dc.description.sponsorshipVytauto Didžiojo universitetas-
dc.format.extentp. 51-
dc.relation.ispartofThe vital nature sign [electronic resource] : 14th international scientific conference, October 15-16, 2020 Kaunas, Lithuania / editors: Nicola Tiso, Vilma Kaškonienė. Kaunas : Vytautas Magnus university, 2020, [no. 14]-
dc.subjectCalcium electroporationen
dc.subjectMicrosecond electroporationen
dc.subjectPI electrotransferen
dc.subjectPore resealingen
dc.subjectMembrane repairen
dc.subject.classificationTezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)-
dc.subject.otherBiofizika / Biophysics (N011)-
dc.titleInduced decrease in small molecule electrotransfer efficiency by extracellular calciumen
dc.typeconference paper-
local.object{"source": {"code": "vdu", "handle": "64503"}, "publisher": {"other": ["Vytautas Magnus university"], "list": false}, "db": {"clarivate": false, "scopus": false, "list": false}, "issn": ["2335-8653"], "code": "T1e", "subject": ["N011"], "country": "LT", "language": "en", "area": "N", "original": true, "pages": 1, "sheets": 0.071, "timestamp": "20201022170256.0", "account": {"year": 2020, "late": false}, "na": 5, "nip": 0, "affiliation": [{"contribution": 0.2, "aip": 1, "country": ["LT"], "rel": "aut", "org": [{"create": true, "contribution": 0.2, "name": "Vytauto Didžiojo universitetas", "id": "111950396", "level": "0", "type": "uni", "research": "1", "status": "1", "unit": {"name": "Gamtos mokslų fakultetas", "id": "01", "level": "1", "type": "fak", "research": "1", "status": "1"}}], "id": "689BF8C2AFADB60B22D8732CC506F3BF", "lname": "Navickaitė", "fname": "Diana", "status": "1", "orcid": "0000-0002-4559-4987", "name": "Navickaitė, Diana"}, {"contribution": 0.2, "aip": 1, "country": ["LT"], "rel": "aut", "org": [{"create": true, "contribution": 0.2, "name": "Vytauto Didžiojo universitetas", "id": "111950396", "level": "0", "type": "uni", "research": "1", "status": "1", "unit": {"name": "Gamtos mokslų fakultetas", "id": "01", "level": "1", "type": "fak", "research": "1", "status": "1", "unit": {"name": "Biologijos katedra", "id": "0102", "level": "2", "type": "kat", "research": "1", "status": "1"}}}], "id": "AB4D82C76EEB275FF3BD707CD43A857A", "lname": "Ruzgys", "fname": "Paulius", "status": "1", "orcid": "0000-0002-4501-4073", "name": "Ruzgys, Paulius"}, {"contribution": 0.2, "aip": 1, "country": ["LT"], "rel": "aut", "org": [{"create": true, "contribution": 0.2, "name": "Vytauto Didžiojo universitetas", "id": "111950396", "level": "0", "type": "uni", "research": "1", "status": "1", "unit": {"name": "Gamtos mokslų fakultetas", "id": "01", "level": "1", "type": "fak", "research": "1", "status": "1", "unit": {"name": "Biologijos katedra", "id": "0102", "level": "2", "type": "kat", "research": "1", "status": "0"}}}], "id": "735B916B25556CCBB883022543FD0BF9", "lname": "Maciulevičius", "fname": "Martynas", "status": "1", "orcid": "0000-0003-1230-6587", "name": "Maciulevičius, Martynas"}, {"contribution": 0.2, "aip": 1, "country": ["LT"], "rel": "aut", "org": [{"create": true, "contribution": 0.2, "name": "Vytauto Didžiojo universitetas", "id": "111950396", "level": "0", "type": "uni", "research": "1", "status": "0", "unit": {"name": "Gamtos mokslų fakultetas", "id": "01", "level": "1", "type": "fak", "research": "1", "status": "0", "unit": {"name": "Biologijos katedra", "id": "0102", "level": "2", "type": "kat", "research": "1", "status": "0"}}}], "id": "4928BF82822D752BDF55752898E8158D", "lname": "Sincevičiūtė", "fname": "Rūta", "status": "0", "name": "Sincevičiūtė, Rūta"}, {"contribution": 0.2, "aip": 1, "country": ["LT"], "rel": "aut", "org": [{"create": true, "contribution": 0.2, "name": "Vytauto Didžiojo universitetas", "id": "111950396", "level": "0", "type": "uni", "research": "1", "status": "1", "unit": {"name": "Gamtos mokslų fakultetas", "id": "01", "level": "1", "type": "fak", "research": "1", "status": "1", "unit": {"name": "Biologijos katedra", "id": "0102", "level": "2", "type": "kat", "research": "1", "status": "1"}}}], "id": "FB0BEA3FB76F9141D78087194DD552D7", "lname": "Šatkauskas", "fname": "Saulius", "status": "1", "orcid": "0000-0002-9001-7767", "name": "Šatkauskas, Saulius"}]}-
item.fulltextWith Fulltext- mokslų fakultetas- katedra- katedra- katedra- katedra-
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications
Files in This Item:
marc.xml6.43 kBXMLView/Open

MARC21 XML metadata

Show simple item record
Export via OAI-PMH Interface in XML Formats
Export to Other Non-XML Formats

CORE Recommender

Page view(s)

checked on Mar 30, 2021


checked on Mar 31, 2021

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.