Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/110198
Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Medicina / Medicine (M001)
Author(s): Lukošiūtė-Urbonienė, Aušra;Mazeikė, Augustina;Kazokaitė, Mintautė;Šilkūnienė, Giedrė;Šilkūnas, Mantas;Barauskas, Vidmantas;Barauskas, Giedrius;Gulbinas, Antanas;Daukša, Albertas;Dambrauskas, Žilvinas
Title: Epigenetic regulation of APAF-1 through DNA methylation in Pancreatic cancer
Is part of: Anticancer research. Attiki, Greece : International Institute of Anticancer Research, 2020, vol. 40, no. 7
Extent: p. 3765-3779
Date: 2020
Keywords: Pancreatic neoplasms;genetics;Apoptotic protease-activating factor 1;genetics;DNA methylation;Decitabine
Abstract: Background/aim: Apoptotic peptidase activating factor 1 (APAF-1) is essential regulator of apoptosis and inactivation by DNA methylation is common event in numerous cancer types. We investigated the regulation of APAF-1 through DNA methylation in pancreatic cancer. Materials and methods: Datasets from 44 patients after pancreatoduodenectomy and the pancreatic adenocarcinoma (PDAC) cell lines Capan-2 and MIA PaCa-2 treated with decitabine were analyzed by RT-PCR, immunoblotting, methylation-specific PCR analysis, apoptosis and viability assays to identify effects of APAF-1 regulation. Results: APAF-1 mRNA and protein levels were significantly down-regulated, and APAF-1 methylation status was associated with perineural invasion in PDAC. Decitabine inhibited cell viability and increased apoptosis rates, however failed to restore APAF-1 mRNA and protein levels in cells. Conclusion: APAF-1 gene hypermethylation may contribute to the progression of PDAC through perineural invasion. Decitabine could sensitize pancreatic cancer cells to apoptosis and growth retardation, however, not directly through the APAF-1 demethylation process
Internet: https://pubmed.ncbi.nlm.nih.gov/32620616/
Affiliation(s): Gamtos mokslų fakultetas
Lietuvos sveikatos mokslų universitetas
Lietuvos sveikatos mokslų universitetas. Medicinos akademija
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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