Please use this identifier to cite or link to this item:
Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Medicina / Medicine (M001)
Author(s): Lukošiūtė-Urbonienė, Aušra;Mazeikė, Augustina;Kazokaitė, Mintautė;Šilkūnienė, Giedrė;Šilkūnas, Mantas;Barauskas, Vidmantas;Barauskas, Giedrius;Gulbinas, Antanas;Daukša, Albertas;Dambrauskas, Žilvinas
Title: Epigenetic regulation of APAF-1 through DNA methylation in Pancreatic cancer
Is part of: Anticancer research. Attiki, Greece : International Institute of Anticancer Research, 2020, vol. 40, no. 7
Extent: p. 3765-3779
Date: 2020
Keywords: Pancreatic neoplasms;genetics;Apoptotic protease-activating factor 1;genetics;DNA methylation;Decitabine
Abstract: Background/aim: Apoptotic peptidase activating factor 1 (APAF-1) is essential regulator of apoptosis and inactivation by DNA methylation is common event in numerous cancer types. We investigated the regulation of APAF-1 through DNA methylation in pancreatic cancer. Materials and methods: Datasets from 44 patients after pancreatoduodenectomy and the pancreatic adenocarcinoma (PDAC) cell lines Capan-2 and MIA PaCa-2 treated with decitabine were analyzed by RT-PCR, immunoblotting, methylation-specific PCR analysis, apoptosis and viability assays to identify effects of APAF-1 regulation. Results: APAF-1 mRNA and protein levels were significantly down-regulated, and APAF-1 methylation status was associated with perineural invasion in PDAC. Decitabine inhibited cell viability and increased apoptosis rates, however failed to restore APAF-1 mRNA and protein levels in cells. Conclusion: APAF-1 gene hypermethylation may contribute to the progression of PDAC through perineural invasion. Decitabine could sensitize pancreatic cancer cells to apoptosis and growth retardation, however, not directly through the APAF-1 demethylation process
Affiliation(s): Gamtos mokslų fakultetas
Lietuvos sveikatos mokslų universitetas
Lietuvos sveikatos mokslų universitetas. Medicinos akademija
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

Files in This Item:
marc.xml8.12 kBXMLView/Open

MARC21 XML metadata

Show full item record
Export via OAI-PMH Interface in XML Formats
Export to Other Non-XML Formats

CORE Recommender

Citations 5

checked on Apr 3, 2021

Page view(s)

checked on Mar 30, 2021


checked on Mar 31, 2021

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.