Use this url to cite publication: https://hdl.handle.net/20.500.12259/110198
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Epigenetic regulation of APAF-1 through DNA methylation in Pancreatic cancer
Type of publication
Straipsnis Web of Science ir Scopus duomenų bazėje / Article in Web of Science and Scopus database (S1)
Author(s)
Lukošiūtė-Urbonienė, Aušra | Lietuvos sveikatos mokslų universitetas |
Mazeikė, Augustina | |
Kazokaitė, Mintautė | Lietuvos sveikatos mokslų universitetas |
Lietuvos sveikatos mokslų universitetas | |
Lietuvos sveikatos mokslų universitetas | |
Barauskas, Vidmantas | Lietuvos sveikatos mokslų universitetas |
Barauskas, Giedrius | Lietuvos sveikatos mokslų universitetas. Medicinos akademija |
Gulbinas, Antanas | Lietuvos sveikatos mokslų universitetas |
Daukša, Albertas | Lietuvos sveikatos mokslų universitetas |
Dambrauskas, Žilvinas | Lietuvos sveikatos mokslų universitetas |
Title
Epigenetic regulation of APAF-1 through DNA methylation in Pancreatic cancer
Is part of
Anticancer research. Attiki, Greece : International Institute of Anticancer Research, 2020, vol. 40, no. 7
Date Issued
Date Issued |
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2020 |
Publisher
Attiki, Greece : International Institute of Anticancer Research
Is Referenced by
Extent
p. 3765-3779
Field of Science
Abstract
Background/aim: Apoptotic peptidase activating factor 1 (APAF-1) is essential regulator of apoptosis and inactivation by DNA methylation is common event in numerous cancer types. We investigated the regulation of APAF-1 through DNA methylation in pancreatic cancer. Materials and methods: Datasets from 44 patients after pancreatoduodenectomy and the pancreatic adenocarcinoma (PDAC) cell lines Capan-2 and MIA PaCa-2 treated with decitabine were analyzed by RT-PCR, immunoblotting, methylation-specific PCR analysis, apoptosis and viability assays to identify effects of APAF-1 regulation. Results: APAF-1 mRNA and protein levels were significantly down-regulated, and APAF-1 methylation status was associated with perineural invasion in PDAC. Decitabine inhibited cell viability and increased apoptosis rates, however failed to restore APAF-1 mRNA and protein levels in cells. Conclusion: APAF-1 gene hypermethylation may contribute to the progression of PDAC through perineural invasion. Decitabine could sensitize pancreatic cancer cells to apoptosis and growth retardation, however, not directly through the APAF-1 demethylation process.
Type of document
type::text::journal::journal article::research article
Language
Anglų / English (en)
Coverage Spatial
Graikija / Greece (GR)