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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biofizika / Biophysics (N011);Biochemija / Biochemistry (N004);Chemijos inžinerija / Chemical engineering (T005)
Author(s): Šilkūnas, Mantas;Bavirša, Mark;Saulė, Rita;Batiuškaitė, Danutė;Saulis, Gintautas
Title: To breathe or not to breathe? Hypoxia after pulsed-electric field treatment reduces the effectiveness of electrochemotherapy in vitro
Is part of: Bioelectrochemistry. Amsterdam : Elsevier Science BV, 2021, vol. 137
Extent: p. 1-13
Date: 2021
Note: Accepted date:16 August 2020; 107636
Keywords: Electroporation;Electrochemotherapy;Activated bleomycin;Hepatocellular carcinoma;Normoxia;Media acclimatization
Abstract: Bleomycin, which is the most widely used drugs in electrochemotherapy, requires oxygen to be able to make single- or double-strand brakes in DNA. However, the concentration of oxygen in tumours can be lower than 1%. The aim of this study was to find out whether oxygen concentration in the medium in which cells loaded with bleomycin are incubated, affects the effectiveness of electrochemotherapy in vitro. Experiments were carried out on mouse hepatoma MH-22A cells. Cells were loaded with bleomycin by using a single square-wave electric pulse (2 kV/cm, 100 μs) under normoxic conditions, seeded into Petri dishes, and grown under normoxic and hypoxic conditions. Cell viability was determined by means of a colony-forming assay. We demonstrated that when cells loaded with bleomycin were incubated in hypoxia (0.2% O2), up to 5.3-fold higher concentrations of bleomycin were needed to kill them in comparison with cells grown in normoxia (18.7% O2)
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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