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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Chemija / Chemistry (N003)
Author(s): Lelešius, Raimundas;Girdauskaitė, P;Karpovaitė, Agneta;Mickienė, Rūta;Drevinskas, Tomas;Tiso, Nicola;Ragažinskienė, Ona;Kubilienė, Loreta;Maruška, Audrius;Šalomskas, Algirdas
Title: An in vitro assessment of antiviral activity for ethanol extract of Desmodium canadense against bovine herpesvirus type 1
Is part of: Polish journal of veterinary sciences. Warszawa: Polish Academia of Sciences, 2020, Vol. 23, iss. 2
Extent: p. 177-184
Date: 2020
Keywords: Bovine herpesvirus 1;Plant extracts;Desmodium canadense
Abstract: Herpesviruses (HV) are pathogens causing infections in humans and animals worldwide. Since it shares many common features with other HV, bovine HV type 1 (BoHV-1) was selected as a model to test the anti-herpesviral activity of medicinal plants. Fifteen plants were chosen in this study for their medical, antibacterial and antiviral properties. The aim was to investigate ethanolic extracts from the selected medicinal plants for anti-BoHV-1 activity. The virucidal activities were evaluated by comparing the effect of noncytotoxic concentrations of extracts on BoHV-1 strain 1640 replication in Madin-Darby bovine kidney (MDBK) cells. Virucidal activity was determined by means of virus titration after exposure to the extracts. The extract of Desmodium canadense was found to be the most effective virucide – the 50% tissue culture infective dose (TCID50) after exposure was 3.75 log10 and the virus reduction factor was ≥5.0±0.25 log10. The extract of D. canadense was therefore chosen for further studies. Virus yield reduction assays showed that D. canadense extract had time-dependent and dose-dependent effects. It effectively reduced virus titre from 8.33 log10 to 4.67 log10 (p<0.01). The virucidal activity was also confirmed by real-time polymerase chain reaction (real-time PCR), where the number of threshold cycles (Ct) was inversely proportional to the virus titre in TCID50 The virucidal activity was also confirmed by real-time polymerase chain reaction (real-time PCR). This method showed that the number of threshold cycles (Ct) was inversely proportional to the virus titre (direct correlation with exposure time R=0.9321). The extract of D. canadense showed a high virus reduction capacity. In future, such active substances should be identified for the development of effective antivirals
Affiliation(s): Biochemijos katedra
Instrumentinės analizės APC
Lietuvos sveikatos mokslų universitetas
Lietuvos sveikatos mokslų universitetas. Veterinarijos akademija
Vaistinių ir prieskon. augalų moks. sek.
Vytauto Didžiojo universitetas
Appears in Collections:1. Straipsniai / Articles
Universiteto mokslo publikacijos / University Research Publications

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