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Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Agronomija / Agronomy (A001);Biochemija / Biochemistry (N004)
Author(s): Blinstrubienė, Aušra;Burbulis, Natalija;Juškevičiūtė, Neringa;Vaitkevičienė, Nijolė;Žūkienė, Rasa
Title: Effect of growth regulators on Stevia rebaudiana Bertoni callus genesis and influence of auxin and proline to steviol glycosides, phenols, flavonoids accumulation, and antioxidant activity In Vitro
Is part of: Molecules. Basel : MDPI AG, 2020, vol. 25, no. 12
Extent: p. 1-15
Date: 2020
Keywords: Antioxidant activity;Callus;Flavonoids;Stevia rebaudiana Bertoni;Phenolic compounds;Rebaudioside A;Stevioside
Abstract: Stevia is a plant containing many active compounds, but usually propagated by stem cuttings because of low seed-yield-germination ability. The aim of this study was to investigate the impact of plant-growth regulators on stevia callus induction and growth from somatic tissue, as well as to determine the effect α-naphthalene acetic acid (NAA) and proline (PRO) on the amount of stevioside, rebaudioside A, phenols, flavonoids, and antioxidant activity. Stem and leaf segments were inoculated on a Murashige and Skoog (MS) medium supplemented with different concentrations of NAA and 6-benzylaminopurine (BAP) for callus genesis. The amount of steviol glycosides (SGs) was evaluated using high-performance liquid chromatography (HPLC), and the amounts of total phenols, flavonoids, and antioxidant activity by spectrophotometric methods. The highest callus-induction frequency and callus-mass increase were obtained from the leaf explants in MS medium supplemented with 2.0 μM NAA. The highest amount of SGs, phenols, and flavonoids, and stronger antioxidant activity were determined in the cellular compounds of callus from leaf explant. PRO reduced the amount of SGs and flavonoids. The significantly highest amount of total phenolic compounds was obtained in the callus from leaf explants in the medium supplemented with 2.0 µM NAA and 2.0 µM PRO
Affiliation(s): Agronomijos fakultetas
Biochemijos katedra
Vytauto Didžiojo universitetas
Appears in Collections:1. Straipsniai / Articles
Universiteto mokslo publikacijos / University Research Publications

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