Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/101545
Type of publication: research article
Type of publication (PDB): Straipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)
Field of Science: Biofizika / Biophysics (N011)
Author(s): Ruzgys, Paulius;Jakutavičiūtė, Milda;Šatkauskienė, Ingrida;Čepurnienė, Karolina;Šatkauskas, Saulius
Title: Effect of electroporation medium conductivity on exogenous molecule transfer to cells in vitro
Is part of: Scientific reports [electronic resource]. London : Nature Publishing Group, 2019, vol. 9
Extent: p. 1-9
Date: 2019
Note: Article number: 1436
Keywords: Membrane electroporation;Gene-transfer;Propidium iodide (PI);Molecule transfer;Electropermeabilization
Abstract: In this study we evaluated the influence of medium conductivity to propidium iodide (PI) and bleomycin (BLM) electroporation mediated transfer to cells. Inverse dependency between the extracellular conductivity and the efficiency of the transfer had been found. Using 1 high voltage (HV) pulse, the total molecule transfer efficiency decreased 4.67 times when external medium conductivity increased from 0.1 to 0.9 S/m. Similar results had been found using 2 HV and 3 HV pulses. The percentage of cells killed by BLM electroporation mediated transfer had also decreased with the conductivity increase, from 79% killed cells in 0.1 S/m conductivity medium to 28% killed cells in 0.9 S/m conductivity medium. We hypothesize that the effect of external medium conductivity on electroporation mediated transfer is triggered by cell deformation during electric field application. In high conductivity external medium cell assumes oblate shape, which causes a change of voltage distribution on the cell membrane, leading to lower electric field induced transmembrane potential. On the contrary, low conductivity external medium leads to prolate cell shape and increased transmembrane potential at the electrode facing cell poles
Internet: https://www.vdu.lt/cris/bitstream/20.500.12259/101545/2/ISSN2045-2322_2019_V_9.PG_1-9.pdf
https://hdl.handle.net/20.500.12259/101545
https://doi.org/10.1038/s41598-018-38287-8
Affiliation(s): Biologijos katedra
Vytauto Didžiojo universitetas
Appears in Collections:1. Straipsniai / Articles
Universiteto mokslo publikacijos / University Research Publications

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