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Type of publication: Straipsnis Clarivate Analytics Web of Science ar/ir Scopus / Article in Clarivate Analytics Web of Science or / and Scopus (S1)
Field of Science: Biofizika / Biophysics (N011)
Author(s): Chopra, Sonam;Ruzgys, Paulius;Jakutavičiūtė, Milda;Rimgailaitė, Aistė;Navickaitė, Diana;Šatkauskas, Saulius
Title: A Novel method for controlled gene expression via combined bleomycin and plasmid DNA electrotransfer
Is part of: International journal of molecular sciences. Basel : MDPI AG, 2019, Vol. 20, iss. 16
Extent: p. 1-12
Date: 2019
Note: Article no. 4047
Keywords: Gene electrotransfer;Cell death;Bleomycin;Plasmid DNA;Transfection;Cytotoxic effect
Abstract: Electrochemotherapy is an e cient method for the local treatment of cutaneous and subcutaneous metastases, but its e cacy as a systemic treatment remains low. The application of gene electrotransfer (GET) to transfer DNA coding for immune system modulating molecules could allow for a systemic e ect, but its applications are limited because of possible side e ects, e.g., immune system overactivation and autoimmune response. In this paper, we present the simultaneous electrotransfer of bleomycin and plasmid DNA as a method to increase the systemic e ect of bleomycin-based electrochemotherapy. With appropriately selected concentrations of bleomycin and plasmid DNA, it is possible to achieve e cient cell transfection while killing cells via the cytotoxic e ect of bleomycin at later time points. We also show the dynamics of both cell electrotransfection and cell death after the simultaneous electrotransfer of bleomycin and plasmid DNA. Therefore, this method could have applications in achieving the transient, cell death-controlled expression of immune system activating genes while retaining e cient bleomycin mediated cell killing
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:1. Straipsniai / Articles
Universiteto mokslo publikacijos / University Research Publications

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