Molecular markers linked to resistance to BRV and Cecidophyopsis ribis in ribes spp
| Author | Affiliation | |||
|---|---|---|---|---|
LT | ||||
Mažeikienė, Ingrida | Lietuvos agrarinių ir miškų mokslų centro filialas Sodininkystės ir daržininkystės institutas | LT | ||
LT | Lietuvos agrarinių ir miškų mokslų centro filialas Sodininkystės ir daržininkystės institutas | LT |
| Date | Issue |
|---|---|
2018 | 12 |
Blackcurrant reversion virus (BRV) belongs to genus Nepovirus. BRV vector is gall mite (Cecidophyopsis ribis) and both phatogens are widespread in all countries where blackcurrants are grown. R. nigrum is the primary natural host of BRV, although natural infestations occur also in other Ribes ssp. Some Ribes species and cultivars are resistant to BRV or C. ribis, and the origin of resistance to virus is not clear. It is known that the resistance to these pathogens is heterogeneous. Several molecular markers related to resistance genes P and Ce have been identified in several Ribes spp. genotypes by scientific researches. Reliable molecular markers are useful for selection of Ribes spp. cultivars with inherited genetic resistance to BRV and C. ribis. The aim of the research: evaluate the resistance of Ribes cultivars and species to BRV virus and gall mite using molecular methods. Methods. The presence of the 7 molecular markers related to resistance to BRV and gall mite in 13 R. nigrum cultivars and 3 Ribes species were established. Markers were searched by PCR and AFLP methods using DNA purified by modified CTAB method. AFLP data were analysed with GeneMapper 4.0 software. Factorial analysis and phylogenetic dendrogram were performed by the DARwin program version 6.0.015. Results. Factorial analysis has shown that tested molecular markers grouped according to the dependence on resistance to C. ribis or BRV. The combination of molecular markers E36/M59-107 and E41/M40-222 identifies the resistance to gall mite, combination of SCAR Ce-130, E41/M43-179, E41/M42-388, E41/M88-280 and E40/M43-236 – resistance to reversion virus. In phylogenetic dendrogram cultivars and species divided into 4 groups, bootstrap 30.0 %. In this research species were used as resistance donors. 46.15 % of cultivars had genetic resistance to gall mite and reversion virus, 53.85 % – were susceptible [...]