Molecular cloning and sequencing of chicken IL–6 CDS and study its comparative genomic analysis with some avian species

Abstract

The Indian poultry industry contributes approximately Rs.22000 crores to the GNP and supports the livelihood of 2.0 million people. But, there is a big gap between the production and per capita availability of the poultry products. It is because of broiler stocks are either plateaued or on the verge of selection limit due to continued long term selection or even losses due to disease. cDNA encoding Chicken IL-6 was cloned from Con A stimulated Chicken Peripheral blood mononuclear cells using oligonucleotide primer..The ChIL-6 cDNA is 710nt long encoding protein of 240 aa. ChIL-6 shows respectively, 95.2%, 86.7%, 75.3% and 55.2% identities with Quail IL-6, DuIL-6, guinea fowl IL-6 and TuIL-6 in cDNA and also shows respectively 93.6% , 92.5% , 57.7% and 2.7%, identities with Turkey IL-6, Quail IL-6, duck IL-6 and Guinea fowl IL-6 in amino acid sequence. This study provides a useful data for further immunological studies of IL-6 in avian immune systems.