Evaluation of synergy of antifungal activity of the secondary metabolites extracted from Eryngium L. species

Abstract

The antifungal activity of methanolic extracts of three Eryngium L. genera (E. planum, E. campestre, E. maritimum) originated from the sector of medicinal plants, botanical garden of Vytautas Magnus University, Lithuania - were tested by the method of series dilutions, against different fungi species. Investigated microorganisms were Aspergillus flavus, Penicillium chrysogenum, Cladosporium cladosporioides, Alternaria alternata, Rhizopus microsporus, Trichoderma viride. The antifungal activities of extracts were described by determination of the Minimal Inhibitory Concentration (MIC). Preliminary results show that the MIC range between 5 %v/v and 20 %v/v of the extracts for the different Eryngium L. extracts and fungi species. The total amounts of phenolic compounds, total amounts of flavonoids and essential oils were tested in the methanolic extracts of the plants. The extracts chemical composition were analyzed by chromatography methods. Synergy of phenolic fraction and essential oils of the plants was determined against the tested fungi. Acknowledgements. Postdoctoral research work fellowship (Dr. Rūta Mickienė) funded by European Union Structural Funds project ”Postdoctoral Fellowship Implementation in Lithuania” (VP1-3.1-ŠMM-01) is highly acknowledged.