Genetic characteristic of morphologic different clones of Vaccinium oxycoccus collested from Žuvintas, Čepkeliai, Kamanos rezerve and Aukštaitija national park

Abstract

The genetic population structure of the wild Cranberry Vaccinium oxycoccus was studied using RAPDs (randomamplified polymorphic DNA). In the last century during intensive peat bogs drainage, regulation of water levels, intensive cranberry picking has caused risk for wild cranberry Vaccinium oxycoccus population’s survival in Lithuania. Genetic variation among and within V. oxycoccus populations with RAPD profiles was investigated. Fifty-six clones were sampled in 4 populations at Čepkeliai, Žuvintas, Kamanos rezerves and Aukštatijos national park. RAPD analyses of 9 primers have shown 213 polymorphic loci in the total sample. Polymorphism level in Čepkeliai was 56.34%, Žuvintas 49.77%, Kamanos 46.95 % and in Aukštatijos national park population 43.19 %. Polymorphism among populations was 100%. The UPGMA analyses have revealed that populations of V.oxycoccus are clearly separated into four lineages and just one Čepkeliai lineage hade homogenous haplotype. Others Žuvintas, Kamanos Aukštatija lineages differed from each other. One clones sampled from Aukštaitijos national park (a8) had unique haplotype.