Auxin effect on in vitro growth of larch endophytic fungus
Date | Volume | Start Page | End Page |
---|---|---|---|
2024 | 18 | 74 | 74 |
Plant hormones are chemical messengers that, acting in micromolar or even smaller concentrations, control plant growth and development. They include auxins, cytokinins, gibberellins, abscisic acid, ethylene, and a few other chemical compounds. Plant hormones are produced not only by plants but also by various microorganisms and fungi [1]; however, the effect of plant hormones on the growth and development of fungi is relatively poorly researched, with data coming only from several fungal species [2-3]. In this study, the effect of auxins indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) was investigated on an endophytic fungus, isolated from surface-disinfected European larch (Larix decidua) shoot buds. The fungus, after being isolated from larch explants, was grown in vitro on an artificial nutrient medium in Petri plates. For experiments, the fungus was planted either on the medium containing auxins (1 μmol/L and 3 μmol/L of IAA or IBA), or on the hormone-free medium shared by apical or lateral larch buds in the same Petri plate. Hormone-free medium without larch explants was used as control in these experiments. The results showed that the growth of fungus was not significantly affected by larch explants planted in the same Petri plate; meanwhile, both auxins had negative effect on fungal growth. However, the effect of IAA was significant only at concentration of 3 μmol L-1; in contrast, both concentrations of IBA significantly diminished the average size of fungal colony on the nutrient medium, with 3 μmol/L having no advantage over 1 μmol/L. The results of this study indicate that auxins can play a role in preventing the outgrowth of plant-colonizing fungi.