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Type of publication: conference paper
Type of publication (PDB): Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biologija / Biology (N010)
Author(s): Armonavičius, Domantas;Maruška, Audrius Sigitas;Drevinskas, Tomas
Title: Genetical identification of intibacterial agents producing microorganisms and analysis of bacteriocins and killer toxins produced by them
Is part of: Vital nature sign [electronic resource] : 13th international scientific conference, May 16-17, 2019, Kaunas, Lithuania : abstract book / editors Nicola Tiso, Vilma Kaškonienė. Kaunas : Vytautas Magnus university, 2019, [no. 13]
Extent: p. 24-24
Date: 2019
Keywords: Bacteriocins;Killer toxins;Antibacterial activity;Capillary electrophoresis
Abstract: Research on naturally occurring antibacterial agents has attracted considerable scientific attention worldwide over the last twenty years. Instead of chemical food additives, it is possible to use natural antimicrobials released by lactic acid bacteria and yeast. The pure antimicrobials produced by microorganisms or the microorganisms that produce these substances can be used for food bio-preservation or as probiotics in medicine and veterinary medicine. Searching for lactic acid bacteria and yeast in natural foods and researching their application possibilities are relevant worldwide. Depending on the environment, microorganisms adapt, resulting in different strains that can produce antibacterial substances with different properties. Such substances are bacteriocins, produced by lactic acid bacteria and killer toxins, produced by yeast [1, 2]. The aim of this work is genotyping of microorganisms, isolated from cottage cheese, obtained from local milk products producers, which shows antibacterial activity against S. aureus, E. coli, M. luteus and P. vulgaris strains. MALDI-TOF MS method was used for this task [3]. Leuconostoc mesenteroides lactic acid bacteria and Kluyveromyces marxianus, Debaromyces hansenii, Candida zeylanoides, Candida inconspicua yeasts were identified during this work. Additional PGR analysis showed that genotyped microorganisms by MALDI-TOF MS method are the same. To analyze what kind of bacteriocins and killer toxins were produced, capillary electrophoresis was used [4]. Detailed results of the work will be presented during the conference
Affiliation(s): Gamtos mokslų fakultetas
Instrumentinės analizės APC
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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