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Type of publication: Straipsnis recenzuojamoje Lietuvos konferencijos medžiagoje / Article in peer-reviewed Lithuanian conference proceedings (P1f)
Field of Science: Agronomija / Agronomy (A001)
Author(s): Aukštakytė, Liucija
Title: Eksplanto tipo įtaka ispaninio šalavijo netiesioginei organogenezei in vitro
Other Title: Explant influence on spanish sage for indirect organogenesis in vitro
Is part of: Jaunasis mokslininkas 2019 [elektroninis išteklius] : studentų mokslinės konferencijos pranešimų rinkinys, 2019 m. balandžio 11 d. / Vytauto Didžiojo universitetas. Žemės ūkio akademija. Agronomijos fakultetas. Akademija, 2019
Extent: p. 75-77
Date: 2019
Abstract: The aim of the research was to identify the impact of explant type and growth regulator to spanish sage calli and shoots formation in vitro. The experiments were carried out in the Labaratory of Agrobiotechnology in the Institute of Biology and Plant Biotechnology in the Faculty of Agronomy at the Aleksandras Stulginskis University (Vytautas Magnus university Agriculture Academy from 2019) in 2017–2018. Spanish sage hypocotyless and stems explants were used during research. The sterile explants were planted to MS medium without, as well as with, different types and concentrations of growth regulators - 1,5 mg l-1 BAP + 0,5 mg l-1 ISR; 2,0 mg l-1 BAP + 0,5 mg l-1 ISR; 3,0 mg l -1 BAP + 0,5 mg l-1 ISR; 0,5 mg l-1 TDZ; 1,0 mg l-1 TDZ; 1,5 mg l-1 TDZ; 2,0 mg l-1 TDZ. The results of this study shows that the concentration of cytokinins and auxins in the growth medium is very important for callus and shoot regeneration. The most intensive hypocotiles and callus induction processes (100 %) were established in the nutritioned medium supplemented with 3,0 mg l-1 BAP + 0,5 mg l-1 ISR
Affiliation(s): Agronomijos fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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