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Type of publication: Straipsnis Clarivate Analytics Web of Science Master Journal List / Article in Clarivate Analytics Web of Science Master Journal List (S2)
Field of Science: Biologija / Biology (N010)
Author(s): Burbulis, Natalija;Kuprienė, Ramunė;Liakas, Vytautas
Title: Somatic embryogenesis and plant regeneration in immature zygotic embryos of Brassica napus
Is part of: Acta universitatis latviensis= Latvijas Universitātes raksti.Biology. , 2007, Vol. 723
Extent: p. 27-35
Date: 2007
Keywords: Age of zygotic embryos;Brassica napus;pH;Somatic embryogenesis
Abstract: The eff ect of zygotic embryo age and pH of media on somatic embryogenesis induction of spring rapeseed was investigated. Immature zygotic embryos cultured on a growth regulator-free Murashige & Skoog medium produced primary somatic embryos without an intervening callus phase. Secondary somatic embryo induction was also possible on hormone-free medium. Similar to direct embryogenesis, the effi ciency of secondary embryogenesis depended on pH of media. Higher pH (5.0) stimulated the proliferation of primary somatic embryos 25 to 26 days aft er pollination of zygotic embryos, while a lower pH (3.5) signifi cantly increased the number of responding primary somatic embryos developed 20 to 21 days aft er pollination of zygotic embryos. Our study showed that primary somatic embryogenesis proceeded at a lower rate than secondary, but the highest number of somatic embryos per responding explant was obtained in primary somatic embryogenesis. Upon transfer to B5 medium, cotyledonary embryos developed into plantlets at a frequency of 7.3 - 30 %
Affiliation(s): Vytauto Didžiojo universitetas
Žemės ūkio akademija
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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