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Type of publication: Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Žūkienė, Rasa;Budreikaitė, Neringa
Title: The role of protein corona and ion dissociation in cytotoxicity mechanism of zinc oxide nanoparticles
Is part of: 15th international conference of the Lithuanian biochemical society, Dubingiai, June 26-29, 2018 : program and abstract book. Vilnius : Lithuanian biochemical society, 2018
Extent: p. 44-45
Date: 2018
Keywords: Protein corona;Cytotoxicity mechanism;Cell viability
ISBN: 9786099603001
Abstract: The past decade has witnessed an exponential growth in nanotechnology of engineered nanomaterials (ENs) on the large scale for both industrial and household purposes. ZnO nanoparticles (NPs) are amongst the most common ENs. ZnO NPs also have a wide range of biomedical applications like drug delivery, anti- cancer, anti-diabetic, antibacterial, antifungal and agricultural properties. Although ZnO NPs are generally recognized as safe (GRAS) by the US Food and Drug Administration it still has the limitation of cytotoxicity which is yet to be resolved. Cytotoxicity of ZnO NPs can be caused by the combined action of plain NPs, protein-coated NPs formed in biological environment and dissociated Zn2+. In this study non-covalent protein coating of ZnO NPs, known as protein corona, was formed from model protein bovine serum albumin (BSA) resulting in ZnO-BSA NPs. The aim of this study was to evaluate the role of protein corona and ion dissociation in cytotoxicity mechanism of ZnO NPs in CHO cells. Different parameters and targets of NPs in the cells were evaluated: NPs uptake, NPs-induced changes in cell viability, proliferation, membrane permeability, mitochondrial amount and potential, ROS and ATP generation. We have demonstrated that low concentrations (0.1-10 μg/ml) of ZnO-BSA and Zn+2 ions had stimulating effect on cell viability. Protein corona enhances ZnO NPs-induced plasma membrane permeabilization for small and large molecules (propidium iodide and lactate dehydrogenase, respectively) but decreases short-(3 h) and long-term (24 h) ZnO NPs cytotoxicity. Interestingly, NPs slightly decrease mitochondrial amount and mitochondrial potential however NPs increase ATP generation by 2-fold. These effects were more pronounced for NPs with protein corona as compared to plain NPs
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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