Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/59720
Type of publication: conference paper
Type of publication (PDB): Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biologija / Biology (N010)
Author(s): Ramonienė, Edita;Krokaitė, Edvina;Jocienė, Lina;Shakeneva, Dinara;Kupčinskienė, Eugenija
Title: Selection and testing of nuclear and plastid DNA markers for genetic diversity researches of Lithuanian populations of Lythrum salicaria L
Is part of: The vital nature sign [electronic resource] : 12th international scientific conference, May 17-18, 2018, Kaunas, Lithuania : abstract book / editors Nicola Tiso, Vilma Kaškonienė. Kaunas : Vytautas Magnus university, 2018, [no. 12]
Extent: p. 37-37
Date: 2018
Note: Online ISSN: 2335-8718
Keywords: ITS;Molecular markers;Lythraceae;cpDNA
Abstract: Lythrum salicaria L. natural habitats in Europe are changing by anthropogenic activity, like a rising salinity in river waters. Consequences caused by water salinity may reflected in L. salicaria genetic diversity. Nevertheless, problem is a fact, that there are a lack of information about genetic researches and tested molecular markers of L. salicaria, only some invasive L. salicaria populations were investigated using AFLP markers in USA. Consequently, the aim of this study is to discover and verify most suitable AFLP, chloroplast DNA, nuclear ribosomal DNA and microsatellites (SSR) primers and their application conditions for genetic analysis of L. salicaria populations. There were used six fluorescent primers for L. salicaria AFLP analysis, as there were used previously in USA and preliminary results revealed EAGC-6-FAM primer was best performing. Twelve pairs of tested SSR primers, previously used for Punica granatum populations, were inappropriate for L. salicaria. Two pairs of universal ribosomal DNA primers (ITS4+ITS5, ITS4+ITS7) and six different plastid DNA markers were applied for L. salicaria individuals, which are prepared for using of L. salicaria DNA sequencing. Plastid DNA markers previously used for Phalaris arundinaceae L., Impatiens glandulifera L. are suitable for Lythrum salicaria, however, primers originaly created for Cucumis sativus L. are improper
Internet: http://vns.microsep.org/wp-content/uploads/2018/05/AbstractbookVNS2018.pdf
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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