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Type of publication: Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Sližytė, Ramunė;Daugelavičius, Rimantas;Gefenas, Vladas
Title: Synthesis and investigation of antibacterial activity of some pyrimidine derivatives
Is part of: The vital nature sign [electronic resource] : 12th international scientific conference, May 17-18, 2018, Kaunas, Lithuania : abstract book / editors Nicola Tiso, Vilma Kaškonienė. Kaunas : Vytautas Magnus university, 2018, [no. 12]
Extent: p. 60-60
Date: 2018
Note: Online ISSN: 2335-8718
Keywords: Pyrimidine derivatives;Antibacterial activity;Antibiotics;Multidrug resistance
Abstract: This study was aimed to investigate possible inhibitory properties of pyrimidine derivatives on Gram-negative Salmonella enterica subsp. enterica ser. Typhimurium and Gram-positive Staphylococcus aureus subsp. aureus bacteria. Broth microdilluting method (Lysogenic broth as medium) was used detecting minimum inhibitory concentration (MIC) with antibiotics tetracycline and chloramphenicol. Fluorimetric method was used for detection of tetracycline fluorescence (λex = 340 nm, λemi = 535 nm) in medium with pyrimidine derivatives. It was found that the pyrimidine derivatives do not exhibit antibacterial properties and have a minimum inhibitory concentration greater than 400 μg/mL. 2-(Phenacylthio)pyrimidine derivatives at relatively high concentrations (400 μg/mL) turbided medium and are not convenient using microdilluting method. Using relatively high 400 μg/mL concentration of 2-hydroxypyrimidines salts tetracycline MIC was increased in case of S. enterica ΔTolC and S. aureus bacteria, but only sodium salts of 2-hydroxypyrimidine and its 5-chloro derivative increased tetracycline MIC in wild strain S. enterica SL1344. Other compounds did not affect tetracycline MIC. The strongest accumulation of tetracycline (compared to control) was determined in the presence oxime, (tio)semicarbazones and chlorophenyloxy derivatives of 2-(phenacylthio)pyrimidines, but there is no explicit relationship between the initial accumulation of tetracycline in bacteria and change of tetracycline MIC. Semikarbazone of 2-(p-chlorophenacylthio)pyrimidine and S. enterica ΔTolC and S. aureus increased the initial accumulation of tetracycline in cells and reduced tetracycline MIC. In the case of the wild S. enterica SL1433 strain, the latter compound, although causing a higher accumulation of tetracycline, does not affect the reduction of tetracycline MIC. All the results will be presented during the conference
Affiliation(s): Biochemijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Švietimo akademija
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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