Use this url to cite publication: https://hdl.handle.net/20.500.12259/59410
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Optimisation of DNA purification using Silica Anionite as a solid phase extraction adsorbent
Type of publication
Tezės kitame recenzuojamame leidinyje / Theses in other peer-reviewed publication (T1e)
Author(s)
LT | ||
LT | ||
Liesienė, Jolanta | Kauno technologijos universitetas | LT |
Kanopka, Arvydas | Vilniaus universitetas | LT |
LT |
Title
Optimisation of DNA purification using Silica Anionite as a solid phase extraction adsorbent
Is part of
The vital nature sign [electronic resource] : 12th international scientific conference, May 17-18, 2018, Kaunas, Lithuania : abstract book / editors Nicola Tiso, Vilma Kaškonienė. Kaunas : Vytautas Magnus university, 2018, [no. 12]
Date Issued
Date Issued |
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2018 |
Publisher
Kaunas : Vytautas Magnus university
Extent
p. 17-17
Field of Science
Abstract
Currently in science there is a growing need of pure and high-quality DNA for experiments[1]. That is why it is important for a DNA purification to be fast, efficient and relatively inexpensive. Therefore, the aim of this study was to create a stationary phase for solid phase extraction (SPE) and purification of DNA. For this work different adsorbents with attached amino groups were used as solid phase for DNA purification using different routes of synthesis of adsorbent [2] and different DNA loading buffers. Three fractions of one sample have been made – fraction of flow through when loading DNA to the column, fraction of flow through when DNA is washed with wash buffer and fraction of flow through of DNA elution. All fractions were tested using spectrometer for DNA concentration and using horizontal gel-electrophoresis to determine the quality of DNA. It has been determined, that sodium acetate buffer of pH 5.0 was the most successful purification with all adsorbent samples as it contained the least of DNA in DNA loading and DNA washing fractions and had significantly higher amount of DNA in elution fraction.
Type of document
type::text::conference output::conference proceedings::conference paper
Language
Anglų / English (en)
Coverage Spatial
Lietuva / Lithuania (LT)
Description
ONLINE ISSN: 2335-8718. The research was granted by Research Council of Lithuania, project No. 09.3.3-LMT-K-712-03-0129