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Type of publication: Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biologija / Biology (N010)
Author(s): Jakutavičiūtė, Milda;Ruzgys, Paulius;Šatkauskas, Saulius
Title: The effect of external DNA on the efficiency of bi-directional small molecule electrotransport
Is part of: The vital nature sign [electronic resource] : 12th international scientific conference, May 17-18, 2018, Kaunas, Lithuania : abstract book / editors Nicola Tiso, Vilma Kaškonienė. Kaunas : Vytautas Magnus university, 2018, [no. 12]
Extent: p. 11-11
Date: 2018
Note: ONLINE ISSN: 2335-8718
Keywords: Eelctroporation;Calcein;DNA;Belomycin
Abstract: Electroporation is a widely used physical method for increasing the cell membrane permeability. After the application of electric pulses, transient electropores are formed, allowing both the leakage of compounds from the cell (electroextraction) and the delivery of small molecules (electrotransfer) and DNA (electrotransfection) to the cell. For certain clinical applications, simultaneous delivery of both small molecules (e.g. anticancer drugs) and DNA could be advantageous. However, the information about the effects of DNA on small molecule transport is very scarce. We investigated the effect externally provided DNA has on the small molecule transport across electroporated cell membrane. For the determination of electroextraction, we used Chinese Hamster Ovary (CHO) cells loaded with green fluorescent calcein dye, and measured the loss of intracellular calcein fluorescence using flow cytometry. For the determination of small molecule electrotransport, we delivered anticancer drug bleomycin to CHO cells, and evaluated the efficiency of bleomycin electrodelivery using clonogenic assay. Our results show that both the outward flux of calcein and the inward flux of bleomycin were positively affected by the presence of extracellular DNA during the application of the electric field. These findings suggest that a combined treatment of chemotherapy and gene therapy might be a feasible approach towards the increase in the treatment efficiency of cancer
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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