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Type of publication: Konferencijų tezės nerecenzuojamuose leidiniuose / Conference theses in non-peer-reviewed publications (T2)
Field of Science: Biologija / Biology (N010)
Author(s): Radzijevskaja, Jana;Levčenkaitė, Simona;Lipatova, Indrė;Sakalauskas, Povilas;Paulauskas, Algimantas
Title: Identification of Wolbachia endosymbiont bacteria in filarial nematodes
Is part of: Smart Bio: international conference, 18-20 May 2017, Kaunas : abstracts book. Kaunas : Vytautas Magnus University, 2017
Extent: p. 89-89
Date: 2017
Keywords: Wolbachia;Endosymbiont;Dirofilaria repens;Nematodes
ISBN: 9786098104424
Abstract: Wolbachia is intracellular endosymbiont bacteria of filarial nematodes and related with the pathogenesis of filarial diseases, in particular in heavy Dirofilaria spp. infections. The aim of this study was to examine Wolbachia sp. in Dirofilaria infected dogs in Lithuania. Blood samples were collected from dogs presented in veterinary clinics of Kaunas. Out of the 183 dog blood samples, 30 samples were positive for Dirofilaria repens. Wolbachia DNA was detected by PCR of 16S rRNA gene using primers wolbF and wolbR. The obtained specific products of 1018 base pairs were considered as a positive result. Wolbachia DNA was detected in 76.6% (23/30) of Dirofilaria positive samples. The two positive samples were sequenced. Sequence analysis of 16S rRNA gene showed 99% similarity with Wolbachia sp. endosymbionts of nematodes
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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