Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/55754
Type of publication: Straipsnis Clarivate Analytics Web of Science Master Journal List / Article in Clarivate Analytics Web of Science Master Journal List (S2)
Field of Science: Biologija / Biology (N010)
Author(s): Radzijevskaja, Jana;Indriulytė, Renata;Paulauskas, Algimantas;Ambrasienė, Daiva;Turčinavičienė, Jurga
Title: Genetic polymorphism study of Ixodes ricinus L. populations in Lithuania using RAPD markers
Other Title: Ixodes ricinus L. genetinio polimorfizmo tyrimai Lietuvos populiacijose, panaudojant AAPD žymenis
Is part of: Acta zoologica Lituanica. Vilnius : Gamtos tyrimų centro Ekologijos institutas, Vol. 15, no. 4 (2005)
Extent: p. 341-348
Date: 2005
Keywords: Ixodes ricinus;Tick;Genetic variability;PCR;RAPD method
Abstract: Atlikti Lietuvoje labiausiai paplitusios erkės Ixodes ricinus - pagrindinio infekcinių ligų, (erkinis encefalitas, Laimo liga, erlichiozė) pernešėjo - genetinio kintamumo tyrimai, panaudojant atsitiktinai amplifikuotos polimorfinės DNR (AAPD) metodą. Erkės buvo surinktosj skirtinguose Lietuvos rajonuose - Vilniaus ir Biržų. Surinktų erkių taksonominė priklausomybė I. ricinus buvo nustatyta naudojant naujus specifinius pradmenis, sukurtus A. Jenkins. 471 I. ricinus DNR pavyzdžių polimorfizmas ištirtas AAPD metodu. Iš dešimties atsitiktinai susintetintų pradmenų, rasti penki produktyviausi, tinkami erkių I.ricinus populiacijų genetinės įvairovės tyrimams. AAPD analizės duomenys rodo, kad I. ricinus erkės surinktos Biržų ir Vilniaus rajonuose pasižymi didele genetine įvairove. Pagal gautus genetinės įvairovės duomenis buvo nustatyti genetiniai skirtumai tarp skirtingų Lietuvos I. ricinus erkių populiacįjų
Ixodes ricinus ticks are common and widespread in Lithuania. They are the main vector in transmission of infectious diseases like tick-bom encephalitis, Lyme borreliosis and human granulocytic ehrlichiosis. The aim of our study was to investigate genetic variability of I. ricinus ticks in Lithuania. I. ricinus ticks were collected from different locations of Lithuania. Data on genetic polymorphism of I. ricinus were used to determinate genetic distances within and among different Lithuanian populations in this species. At first, 103 collected ticks were analysed using specifics for I. ricinus new primers designed by A. Jenkins for taxonomic identification. PCR analysis data showed that all ticks were I. ricinus. Polymorphism of I. ricinus was analysed using the random amplified polymorphic DNA (RAPD) method. 47 DNA samples of I. ricinus ticks were used for RAPD reaction. Ten arbitrary sequence decamers were used as primers. Reproducible amplification pattems were obtained using five primers. RAPD analysis data showed that I. ricinus from Biržai and Vilnius were highly polymorphic. Similarities between RAPD pattems were used to evaluate genetic distances between I. ricinus populations in Lithuania
Internet: http://www.tandfonline.com/toc/tzec19/15/4#.U5W4YeneiDY
http://www.tandfonline.com/toc/tzec19/15/4#.U5W4YeneiDY
Affiliation(s): Vilniaus universitetas, jurga.turcinaviciene@gf.vu.lt
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

Files in This Item:
marc.xml15.23 kBXMLView/Open

MARC21 XML metadata

Show full item record

Page view(s)

126
checked on Sep 5, 2019

Download(s)

6
checked on Sep 5, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.