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Type of publication: Tezės kituose recenzuojamuose leidiniuose / Theses in other peer-reviewed publications (T1e)
Field of Science: Biologija / Biology (N010)
Author(s): Galdikas, Matas;Paulauskas, Algimantas;Radzijevskaja, Jana;Galdikaitė, Eglė;Mažeika, Vytautas;Stanko, Michail;Petko, B;Karbowiak, Grzegorz
Title: Investigation of genetic diversity of Dermacentor reticulatus ticks in Europe using microsatellite genetic markers
Is part of: German society for parasitology : 27th annual meeting, 9–12 March 2016, Göttingen, Germany : program and abstracts. Göttingen : University of Göttingen, 2016
Extent: p. 152-152
Date: 2016
Keywords: Ticks;Tick-borne diseases;Microsatellite genetic markers
Abstract: Dermacentor reticulatus ticks are important vectors of tick-borne diseases in humans, livestock and wild animals in Europe. Geographic distribution of these ticks is fragmented, usually divided into two groups: Western Europe and Eastern Europe with extension to Asia. In this study we applied fourteen microsatellite markers previously developed for D. variabilis, D. albipictus and D. andersoni in USA. All loci amplified with varying success in D. reticulatus, and six of them were selected for further analyses, based on reliable amplification of the expected fragment length and presence of polymorphism. Polymorphic microsatellites were further characterized in 173 adult D.reticulatus individuals collected from 17 locations along the distribution range of D. reticulatus in Eastern (Latvia, Lithuania, Eastern Poland, Slovakia, Croatia and Ukraine) and Western Europe (Spain and Czech Republic), using capillary electrophoresis. Results of analysis showed that most of the molecular variability is contained within populations, and that populations cluster together similarly to known geographic distribution. All unique alleles were found in Spain and Czech Republic populations. Bayesian-clustering and PCoA analyses showed two genetic groups of D. reticulatus ticks: Western Europe and Eastern Europe. Eastern Europe group showed no evidence of clusterization according to geographical distribution patterns with low molecular variability between populations. Low genetic differences between Lithuanian, Latvian, Poland and Slovak populations of D. reticulatus show close relatedness among these populations
Affiliation(s): Biologijos katedra
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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