Use this url to cite publication: https://hdl.handle.net/20.500.12259/49379
Options
Genetic analysis of Impatiens glandulifera populations using ISSR markers
Type of publication
Tezės kitame recenzuojamame leidinyje / Theses in other peer-reviewed publication (T1e)
Title
Genetic analysis of Impatiens glandulifera populations using ISSR markers
Is part of
The vital nature sign [elektroninis išteklius] : 7-th international scientific conference : abstract book. Kaunas : Vytautas Magnus university, 2013, [no. 7]
Date Issued
Date Issued |
---|
2013 |
Publisher
Kaunas : Vytautas Magnus university
Extent
p. 48-48
Field of Science
Abstract
Impatiens glandulifera is annual weed that came from West of Himalays and nowadays is highly invasive in almost whole Europe and occurs in various habitats. As a result of this fact Impatiens glandulifera displace native species and occupies growing areas near the rivers. Population genetic data on genetic peculiarities may aid in the management of the invasive species. We collected Impatiens glandulifera plants from 13 different abitats located in Lithuania and in the other European countries: Holland, Belgium, Luxembourg, Germany (Heepen, Rinteln, Bad Zalzuflen, Seeker, Switzerland, Austria, Hungary and Czech Republic. These populations we compared using an intersimple sequence repeat (ISSR) markers: ISSR2, ISSR3, ISSR4 and ISSR5. PCR products were separated by 1.5% agarose gel, photographed and statistically analyzed. Fragment length was measured by Gene Ruller (100 bp length standards). Among populations of Impatiens glandulifera genetic parameters ranged in the following intervals: percentage of polymorphic DNA within populations was between 5.6% (Switzerland and Lithuania) and 30.3 % (Germany, Bad Zalzuflen), mean being – 16.1%. In accordance with Nei’s gene diversity index, the most different were northern Luxembourg and Lithuanian populations (0.570), and the most simillar were two populations from Germany (Bad Zalzuflen and Rinteln populations; 0.831). It was found genetic difference between populations – 57%, within population 19% and 24% of the molecular diversity within countries. The principle coordinate analyses of populations and genetic tree built on the bases of UPGMA cluster analyses showed relations to geographical position of sampling sites only for part of populations. It might be explained by the fact that Himalayan balsam is spreading not only naturally - along streams, ditches, but it is unintentionally and intentionally distributed by the human in different distances and directions.
Type of document
type::text::conference output::conference proceedings::conference paper
Language
Anglų / English (en)
Coverage Spatial
Lietuva / Lithuania (LT)