Please use this identifier to cite or link to this item:https://hdl.handle.net/20.500.12259/47505
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dc.contributor.authorKrupovič, Mart-
dc.contributor.authorDaugelavičius, Rimantas-
dc.contributor.authorBamford, Dennis H-
dc.coverage.spatialGB-
dc.date.accessioned2018-10-06T21:45:24Z-
dc.date.available2018-10-06T21:45:24Z-
dc.date.issued2007-
dc.identifier.issn0950382X-
dc.identifier.otherVDU02-000008367-
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-2958.2007.05769.x-
dc.identifier.urihttps://doi.org/10.1111/j.1365-2958.2007.05769.x-
dc.description.abstractIn this study we investigated the lysis system of the lipid-containing double-stranded DNA bacteriophage PM2 infecting Gram-negative marine Pseudoalteromonas species. We analysed wt and lysis-deficient phage-induced changes in the host physiology and ascribed functions to two PM2 gene products (gp) involved in lysis. We show that bacteriophage PM2 uses a novel system to disrupt the infected cell. The novelty is based on the following findings: (i) gp k is needed for the permeabilization of the cytoplasmic membrane and appears to play the role of a typical holin. However, its unique primary structure [53 aa, 1 transmembrane domain (TMD)] places it into a new class of holins. (ii) We have proposed that, unlike other bacteriophages studied, PM2 relies on lytic factors of the cellular origin for digestion of the peptidoglycan. (iii) gp l (51 aa, no TMDs) is needed for disruption of the outer membrane, which is highly rigidified by the divalent cations abundant in the marine environment. The gp l has no precedent in other phage lytic systems studied so far. However, the presence of open reading frame l-like genes in genomes of other bacterial viruses suggests that the same system might be used by other phages and is not unique to PM2en
dc.description.sponsorshipVilniaus universitetas-
dc.format.extentp. 1635-1648-
dc.language.isoen-
dc.relation.ispartofMolecular microbiology. Oxford : Wiley-Blackwell, 2007, vol. 64, no. 6-
dc.relation.isreferencedbyScience Citation Index Expanded (Web of Science)-
dc.relation.isreferencedbyWiley Online Library-
dc.relation.isreferencedbyAcademic Search Premier (EBSCO)-
dc.relation.isreferencedbyMEDLINE-
dc.relation.isreferencedbyIngenta Connect-
dc.subject.classificationStraipsnis Clarivate Analytics Web of Science / Article in Clarivate Analytics Web of Science (S1)-
dc.subject.otherBiologija / Biology (N010)-
dc.subject.otherBiochemija / Biochemistry (N004)-
dc.titleA novel lysis system in PM2, a lipid-containing marine double-stranded DNA bacteriophageen
dc.typeresearch article-
dc.identifier.doihttps://doi.org/10.1111/j.1365-2958.2007.05769.x-
dc.identifier.isiWOS:000247175700019-
dcterms.bibliographicCitation51-
dc.date.updated2020-02-19T15:45Z-
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local.typeS-
item.fulltextNo Fulltext-
item.grantfulltextnone-
crisitem.author.deptBiochemijos katedra-
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications
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