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Type of publication: Straipsnis recenzuojamoje Lietuvos tarptautinės konferencijos medžiagoje / Article in peer-reviewed Lithuanian international conference proceedings (P1e)
Field of Science: Biochemija / Biochemistry (N004)
Author(s): Bimbiraitė, Kristina;Kornyšova, Olga;Maruška, Audrius;Proscevičius, Juozas
Title: Solid phase microextraction followed by gas chromatography – mass spectrometry for the analysis of the volatile compounds from plants
Is part of: The Vital Nature Sign : 3rd International Conference, Kaunas, Lithuania, May 22-23, 2009 : proceedings. Kaunas : Vytautas Magnus university, 2009
Extent: p. 169-172
Date: 2009
Keywords: Phase microextraction;Gas chromatography;Volatile compounds;Plants
ISBN: 9789955125426
Abstract: In this study volatile compounds in initial diploidal (control) and experimentally prepared polyploidal forms of medicinal plants were evaluated. Three annual medicinal plants were selected: fenugreek (Trigonella foenum-graecum L.), milk thistle (Silybum marianum L.) and marigold (Celendula officinalis L.). It was expected that polyploidal forms accumulate higher amount of biologically active compounds than initial diploidal forms. The samples grown in vitro were dried at 30°C. Headspace solid phase microextraction (SPME) was performed with a fiber coated with a 65 μm thick film of PDMS-DVB. Extraction conditions were following: extraction temperature: 40 °C, equilibration time: 15 min; extraction time: 15 min. After sampling, the SPME fiber was inserted into the GC injector port and the essential oils thermally desorbed. A desorption time of 1 min at 230 °C was used in splitless injection mode. Preliminary results have shown that only Silybum marianum L. polyploidal form accumulates higher amount of essential oils comparing with initial diploidal, for Calendula officinalis L. and Trigonella foenum-graecum L. the amount of essential oils was higher in control samples (initial diploids). Additional experiments are needed to confirm the obtained results and special attention should be paid on the drying conditions of the in vitro grown raw material
Affiliation(s): Biochemijos katedra
Botanikos institutas
Gamtos mokslų fakultetas
Vytauto Didžiojo universitetas
Appears in Collections:Universiteto mokslo publikacijos / University Research Publications

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